循环血液外泌体miR145、miR-200与非小细胞肺癌患者放化疗后复发、转移的关系

Correlation of Circulating Exosomal miR-145 and miR-200 with the Recurrence and Metastasis in Patients with Non-small Cell Lung Cancer after Radiotherapy and Chemotherapy

目的 探讨循环血液外泌体微小RNA-145(miR145)、微小RNA-200(miR-200)与非小细胞肺癌患者放化疗后复发、转移的关系。方法 回顾性选取72例非小细胞肺癌患者作为实验组,并选取同期72例健康体检者作为对照组。实验组患者均接受放化疗治疗,出院后展开为期1年的随访,对比2组患者循环血液外泌体miR145、miR-200表达量,分析实验组患者不同病理特征miR145、miR-200表达量;以KM曲线法绘制复发转移曲线分析miR145、miR-200表达量对患者放化疗后复发、转移的影响。结果 与对照组相比,实验组miR145水平显著更低,miR-200显著更高,差异具有统计学意义(P<0.05)。实验组循环血液外泌体miR145和miR-200表达量在不同TNM分期和淋巴结转移情况方面差异比较具有统计学意义(P<0.05);高miR145组(n=42)与低miR145组(n=30)2年内复发转移率分别为28.57%(12/42)和73.33%(22/30),两者差异具有统计学意义(P<0.05);高miR-200组(n=27)与低miR-200组(n=45)2年内复发转移率分别为70.37%(19/27)和33.33%(15/45),两者差异具有统计学意义(P<0.05)。结论 循环血液外泌体miR145、miR-200在非小细胞肺癌患者中出现明显变化,其表达水平与放化疗后复发、转移存在明显的关系。

Objective To explore the correlation of circulating exosomal microRNA-145(miR-145)and microRNA-200(miR-200)with the recurrence and metastasis in patients with non-small cell lung cancer after radiotherapy and chemotherapy.Methods 72 patients with non-small cell lung cancer and 72 healthy subjects were retrospectively analyzed,and set as the experimental group and the control group,respectively.All patients in the experimental group received radiotherapy and chemotherapy,and were followed up for one year after discharge.Circulating miR-145 and miR-200 levels were compared between the 2 groups,and comparison was also conducted among patients of different pathological characteristics.Then Kaplan Meier's survival curve was plotted to analyze the effect of miR-145 and miR-200 expression on the recurrence and metastasis of patients after radiotherapy and chemotherapy.Results Compared with the control group,miR-145 expression was down-regulated and miR-200 expression was up-regulated in the experimental group(P<0.05).Circulating exosomal miR-145 and miR-200 levels yielded statistical difference among patients of different TNM stages and lymph node metastasis(P<0.05).The 2-year recurrence and metastasis rate was 28.57%(12/42)in high miR-145 group(n=42)and was 73.33%(22/30)in low miR-145 group(n=30),with statistical difference(P<0.05).The 2-year recurrence and metastasis rate was 70.37%(19/27)in high miR-200 group(n=27)and was 33.33%(15/45)in low miR-200 group(n=45),with statistical difference(P<0.05).Conclusion An notable change is detected in circulating exosomal miR-145 and miR-200 levels in patients with non-small cell lung cancer,and their expression levels are significantly correlated with recurrence and metastasis in patients after radiotherapy and chemotherapy.