互叶醉鱼草茎段组织培养技术研究

Study on tissue culture of stem segments from Buddleja alternifolia

以互叶醉鱼草带腋芽的茎段为试验材料,采用正交试验设计和组织培养技术,对外植体的消毒时间和腋芽诱导、继代增殖、生根培养及炼苗移栽等技术进行了研究。结果表明:先用75%酒精处理30 s,再使用1%NaClO溶液消毒12 min,外植体存活率最高,达86.7%;初代诱导腋芽最佳培养基为MS+1.0 mg/L 6-BA+0.4 mg/L NAA,诱导率为93.3%;最佳增殖培养基为MS+1.0 mg/L 6-BA+0.3 mg/L NAA+0.3 mg/L IBA,增殖系数为6.47;最佳生根培养基为1/2 MS+0.3 mg/L IBA,生根率为76.7%;将组织培养得到的幼苗栽植到由营养土、蛭石和珍珠岩(容积比为4∶2∶1)组成的混合基质中,成活率为88.3%。

Taking the stem segments with the axillary buds of Buddleja alternifolia as explants,by orthogonal test design and tissue culture techniques,the disinfection time,axillary bud induction,proliferation,rooting culture and seedlings transplanting were studied.The results showed that:treated with 75%alcohol for 30 s,and then disinfected with 1%NaClO solution for 12 min,the explants could obtain the highest survival(up to 86.7%).The optimal medium for the axillary bud induction was claimed as MS+1.0 mg/L 6-BA+0.4 mg/L NAA,with the induction rate up to 93.3%.The optimal proliferation medium was determined as MS+1.0 mg/L 6-BA+0.3 mg/L NAA+0.3 mg/L IBA,with the proliferation coefficient up to 6.47.The optimal rooting medium as 1/2 MS+0.3 mg/L IBA,with the rooting rate of 76.7%.The survival of the plantlets transplanted into the complex substrate composed of nutrient soil,vermiculite,perlite(volume ratio 4∶2∶1),could get up to 88.3%.