共培养策略在类器官研究中的应用进展

Advances in the application of co-culture strategies in organoids

类器官作为一种良好的体外研究模型,在生物医学领域中的应用越来越广泛。通过采用各种组织培养技术开发自组装的3D结构,类器官可重现器官固有结构中细胞的高度复杂性,因此被广泛用于研究调节机体发育和疾病的机制、高通量药物筛选及个性化治疗等。为更好地重现微环境内细胞间的相互作用,共培养策略已经扩展到更多的细胞类型,共培养策略的迅速发展使类器官技术的应用前景更加广阔,并为治疗人类疾病和再生医学开辟了新的道路。本文阐述共培养策略在类器官生成中的作用,并重点介绍不同细胞成分及微生物组与类器官共培养的应用,以期为构建开发具有更高体内模拟程度的类器官提供参考和帮助。

As a good in vitro research model,organoids are more and more widely used in the biomedical field.By developing self-assembled 3D structures using various tissue culture techniques,organoids can rebuild the high complexity of cells in the inherent structure of the organ,and are therefore unanimously used to study mechanisms regulating body development and disease,high-throughput drug screening,and personalized treatment and so on.To better recapitulate cell-to-cell interactions within the microenvironment,co-culture strategies have been extended to more cell types,and their rapid development offers broader prospects for organoids and paves the way for the treatment of human diseases and regenerative medicine.This review discussed the role of co-culture strategies in organoid generation,and focused on the application of various cellular components and microorganisms in organoid construction,thereby providing reference and help for scholars to construct and develop organoids with a higher degree of in vivo simulation.