超高效液相色谱法分析淡豆豉配方颗粒中3种异黄酮

Determination of three isoflavonoids in Sojae Semen Praeparatum formula granules by UPLC

建立超高效液相色谱法同时测定淡豆豉配方颗粒中3种异黄酮(大豆苷元、黄豆黄素、染料木素)的含量。选择Eclipse Plus C18RRHD色谱柱(100 mm×2.1 mm,1.8μm),流动相为乙腈-0.1%乙酸溶液,梯度洗脱,流量为0.3mL/min,柱温为30℃,检测波长为260 nm。大豆苷元、黄豆黄素、染料木素的质量浓度分别在18.41~184.14、0.48~4.82、30.65~306.46μg/mL范围内线性关系良好(r≥0.999 9)。样品测定结果的相对标准偏差分别为1.80%、2.20%、1.71%(n=6),平均加标回收率分别为96.49%、100.93%、98.53%。该方法快速,准确、可靠,可为淡豆豉配方颗粒的质量控制提供参考。

A UPLC(Ultra-high performance liquid chromatography)method was established for the simultaneous determination of three isoflavonoids(daidzein,glycitein and genistein)in Sojae Smen Praeparatum formula granules.The analysis was performed on an Eclipse Plus C18 RRHD column(100 mm×2.1 mm,1.8μm),with the mobile phase comprising of acetonitrile-0.1%acetic acid flowing at 0.3 mL/min in a gradient elution manner,and the detection wavelength was set at 260 nm.The mass concentration of daidzein,glycitein and genistein showed good linear relationships in the ranges of 18.41-184.14,0.48-4.82,and 30.65-306.46μg/mL(r≥0.9999),respectively.The relative standard deviations of the detection results were 1.80%,2.20%,1.71%(n=6),and the average recoveries were 96.49%100.93%,98.53%for daidzein,glycitein and genistein,respectively.This rapid,feasible,accurate and reliable method can be used for the quality control of Sojae Smen Praeparatum formula granules.