玉米ZmHY5和ZmHY5L基因的克隆及其转录丰度对不同光质处理的响应

Cloning of Maize ZmHY5 and ZmHY5L Genes and Their Transcriptional Abundance in Responses to Different Light Treatments

为了探索玉米(Zea mays)HY5(Elongated hypocotyl 5)基因的功能,以玉米自交系B73为材料克隆了2个HY5基因,分别命名为ZmHY5和ZmHY5L。利用实时荧光定量PCR(qRT-PCR)方法分析ZmHY5和ZmHY5L基因在玉米不同组织中的表达量,以及对不同光质、转换光和长短日照处理的响应。结果表明,玉米与高粱、南荻、谷子及拟南芥的HY5具有相似的结构域和较高的氨基酸序列一致性,意味着它们可能具有类似的功能。ZmHY5和ZmHY5L基因的组织表达模式类似,均在叶片中的表达量最高,但整体上ZmHY5基因表达量明显高于ZmHY5L基因;两者对不同持续光质的响应模式也基本类似,ZmHY5基因在蓝光条件下表达量最高,对黑暗转白光的响应更明显。ZmHY5L基因在长日照条件下的响应能力强于ZmHY5基因,而ZmHY5基因在短日照条件下具有更强的黑暗阶段响应能力。暗示ZmHY5和ZmHY5L基因可能在玉米光形态建成及花期调控中发挥作用。

In order to explore the function of maize(Zea mays)HY5(elongated hypocotyl 5)gene,two HY5 genes were cloned from maize inbred line B73,named ZmHY5 and ZmHY5L,respectively.Real⁃time quantitative PCR(qRT⁃PCR)was used to analyze the expression levels of ZmHY5 and ZmHY5L genes in different tissues of maize and their transcriptional abundance in responses to different light,conversion light and long and short sunshine treatments.The results showed that HY5 of Zea mays had similar domains and high amino acid sequence identity with those of Sorghum bicolor,Miscanthus lutarioriparius,Setaria italica and Arabidopsis thaliana,suggesting that they may have similar functions.The tissue expression patterns of ZmHY5 and ZmHY5L genes were similar,with the highest expression level in leaves,but the expression level of ZmHY5 gene was significantly higher than that of ZmHY5L gene.The response patterns of ZmHY5 and ZmHY5L genes to different continuous light were basically similar.ZmHY5 gene had the highest expression level under blue light condition,and more obvious response to darkness to white light.The response ability of ZmHY5L gene under long⁃day condition was stronger than that of ZmHY5 gene,while ZmHY5 gene had stronger response ability in darkness phase under short⁃day condition.Overall,ZmHY5 and ZmHY5L genes may play a role in maize photomorphogenesis and flowering regulation.