综合果蔬酵素发酵工艺优化及其润肠通便功能评价

Optimization of Fermentation Technology of Integrated Fruit and Vegetable Enzyme and Evaluation of Its Function of Nourishing Bowel and Defecating

目的:确定综合果蔬酵素发酵工艺,并探究其润肠通便功能。方法:通过调整发酵时间、发酵温度、低聚异麦芽糖添加量、燕麦麸皮添加量,以感官评分为评价指标,优化综合果蔬酵素的制备工艺,测定酵素产品的特征性成分,建立小鼠便秘模型,探究综合果蔬酵素不同剂量(低剂量5 mL/kg,中剂量10 mL/kg,高剂量15 mL/kg)对小鼠小肠墨汁推进率和排便的影响。结果:应用响应面法优化出综合果蔬酵素的配方为发酵时间为72 h,发酵温度为37℃,低聚异麦芽糖添加量为3%,燕麦麸皮添加量为3%,在此条件下感官评分为95.33分。特征性成分检测中综合果蔬酵素中富含1100 mg/kg乳酸、γ-氨基丁酸100 mg/kg、120 mg/100 g的游离氨基酸,小鼠试验中,高剂量的综合果蔬酵素的墨汁推进率高达66.7%,与对照组相比极显著(P<0.01)提升,6 h内排便粒数高剂量组达到33.16粒,极显著(P<0.01)高于模型对照组,有效缩短了粪便在小肠内的滞留时间,起到润肠通便的作用。结论:优化后的综合果蔬酵素具有较好的感官评分和润肠通便作用,具有一定的应用前景。

Objective:To determine the fermentation technology of dietary fiber enzyme,and to explore its function of nourishing intestine and defecating.Methods:By adjusting the fermentation time,fermentation temperature,the amount of isomaltose and oat bran,the sensory score of integrated fruit and vegetable enzyme was optimized,the characteristic components of the enzyme products were determined.To establish a constipation model in mice,and to explore the effects of different doses of Integrated fruit and vegetable enzymes(low dose 5 mL/kg,medium dose 10 mL/kg,high dose 15 mL/kg)on small intestinal ink propulsion rate and defecation in mice.Results:The formula of integrated fruit and vegetable enzyme was optimized by response surface method.The fermentation time was 72 h,the fermentation temperature was 37℃,the amount of isomaltose added was 3%,and the amount of oat bran added was 3%.In the mouse experiment,the ink advance rate of high-dose integrated fruit and vegetable enzyme was as high as 66.7%,compared with the control group,it was significantly improved(P<0.01),and the number of defecation grains within 6 h reached 33.16 in the high-dose group,it was significantly higher than the model control group(P<0.01),which effectively shortened the retention time of feces in the small intestine,and played the role of moistening the intestine.Conclusion:The optimized integrated fruit and vegetable enzyme of fruits and vegetables has better sensory score and nourishing bowel function,so it has certain application prospect.