摘要
Castor bean(Ricinus communis L.),is one of the top 10 oilseed crops in the world and,therefore,of high economic value.Hybridization is one of the most effective ways to breed new varieties with high yield,high oil content,and better stress resistance.Therefore,prediction of desired traits in castor hybrid offspring is particularly important.In this study,proteomic analysis was performed to identify differentially expressed proteins(DEPs)in seeds between castor hybrid offspring and their female(Lm female line aLmAB2)and male parents(CSR·181).Among the DEPs upregulated in the seeds of hybrid offspring,the majority were related to seed yield and stress tolerance,while some were related to oil synthesis and fatty acid synthesis and metabolism in seeds.In other words,the hybrid offspring showed heterosis for seed yield,stress tolerance,oil synthesis,and fatty acid synthesis and metabolism when compared with their parents.Further,real-time quantitative polymerase chain reaction assays were performed on 12 genes encoding DEPs involved in oil synthesis,pollen abortion,yield,and stress tolerance of seeds.The results showed that the expression levels of the 12 genes were consistent with those of the DEPs.
基金
National Natural Science Foundation of China(31860071)
Research and Reform Practice Project in New Agricultural Sciences of the Ministry of Education in 2020(2020114)
Natural Science Foundation of Inner Mongolia Autonomous Region(2021MS03008)
Inner Mongolia Autonomous Region Grassland Talents Innovation Team-Castor Molecular Breeding Research Innovative Talent Team Rolling Support Project(2022)
Higher Education Teaching Reform Research Project of National Ethnic Affairs Commission in 2021(21082)
Fundamental Research Funds in Higher Education Institutions of Inner Mongolia in 2022(237)
Autonomous Region Basic Scientific Research Business Fee Project of Inner Mongolia Minzu University in 2023(225,227,244)
Inner Mongolia Autonomous Region Castor Industry Collaborative Innovation Center Construction Project(MDK2021011,MDK2022014).