PCR检验法和细菌培养法在阴道细菌检验中的效果对比

Comparison of PCR test and bacterial culture in vaginal bacteria testing

目的分析在阴道细菌检验中选用聚合酶链反应(PCR)检验法和细菌培养法两种手段所呈现出的不同效果。方法50例阴道细菌感染性疾病患者,分别进行PCR检验法、细菌培养法检验,对比两种检验方式的阳性检出率、各病原菌阳性检出率、患者认可度。结果PCR检验法阳性检出率88.00%高于细菌培养法的62.00%,差异有统计学意义(P<0.05)。PCR检验法对加特纳菌的阳性检出率为59.09%,高于细菌培养法的35.48%,差异有统计学意义(P<0.05)。两种检验方式对棒状杆菌、肠球菌阳性检出率对比,差异无统计学意义(P>0.05)。患者对PCR检验法的总认可度为98.00%,高于细菌培养法的76.00%,差异有统计学意义(P<0.05)。结论在阴道细菌检验中选用PCR检验法相较于细菌培养法的检验效果更佳,尤其是在加特纳菌检验中效果突出,可为临床诊治提供重要参考依据,值得推广使用。

Objective To analyze the difference effect of PCR test and bacterial culture in vaginal bacteria testing.Methods 50 patients with vaginal bacterial infectious diseases were tested by PCR and bacterial culture respectively,and the positive detection rate,positive detection rate of each pathogen and patient recognition of the two test methods were compared.Results The positive detection rate by PCR test was 88.00%,which was higher than that of 62.00%by bacterial culture,and the difference was statistically significant(P<0.05).The positive detection rate of PCR test for Gardnerella vaginalis was 59.09%,which was higher than 35.48%of bacterial culture,and the difference was statistically significant(P<0.05).There was no statistically significant difference in the positive detection rate of Corynebacterium and Enterococcus between the two testing methods(P>0.05).The patient's total acceptance for PCR test was 98.00%,which was higher than 76.00%for the bacterial culture,and the difference was statistically significant(P<0.05).Conclusion In vaginal bacterial testing,the PCR test is more effective than the bacterial culture,especially in the detection of Gardnerella vaginalis,which can provide an important reference basis for clinical diagnosis and treatment and is worthy of promotion and application.