IVF/ICSI助孕后早期流产患者蜕膜组织焦亡相关因子表达水平的研究

Study on the expression of pyrolysis-related factors in the decidua tissue of patients with early abortion after IVF/ICSI assisted pregnancy

目的 探讨体外受精(IVF)/卵胞浆内单精子注射(ICSI)助孕妊娠后早期流产患者蜕膜组织中焦亡相关因子的表达及作用。方法 选取行IVF/ICSI助孕妊娠后早期流产患者102例(研究组),同期因意外妊娠行人工流产术的患者100例(对照组),收集蜕膜组织。实时荧光定量PCR(qRT-PCR)法及Western blot法检测蜕膜组织中焦亡相关因子Nod样受体蛋白3(NLRP3)、白细胞介素(IL)-1β、胱天蛋白酶1(Caspase1)、gasdermin D(GSDMD)mRNA及蛋白表达,TUNEL法检测蜕膜组织中细胞焦亡情况,Pearson法分析蜕膜组织中细胞焦亡率与NLRP3、IL-1β、Caspase1、GSDMD蛋白水平的相关性。结果 与对照组相比,研究组蜕膜组织中NLRP3、IL-1β、Caspase1、GSDMD mRNA及蛋白水平、蜕膜组织中细胞焦亡率升高(P<0.05);研究组患者蜕膜组织中细胞焦亡率与NLRP3、IL-1β、Caspase1、GSDMD蛋白表达水平均呈正相关(r分别为0.429、0.474、0.498、0.540,均P<0.05)。结论 IVF/ICSI助孕妊娠后早期流产患者蜕膜组织中焦亡相关因子NLRP3、IL-1β、Caspase1、GSDMD表达均升高,且与蜕膜组织细胞焦亡有关。

Objective To investigate the expression and effect of pyrodeath related factors in decidual tissue of in vitro fertilization(IVF)/intracytoplasmic sperm injection(ICSI)assisted early postpregnancy abortion.Methods A total of 102 patients with early abortion after IVF/ICSI assisted pregnancy were selected as the research group,and 100 patients who underwent artificial abortion due to unexpected pregnancy during the same period were selected as the control group.Samples of decidua tissue were collected.Quantitative real-time PCR(qRT-PCR)method and Western blot assay were used to detect expression levels of Nod-like receptor pyrin domain 3(NLRP3),interleukin-1β(IL-1β),Cysteinyl aspartate specific proteinase 1(Caspase1),gasdermin D(GSDMD)mRNA and protein in decidua tissue.TUNEL method was used to detect the cell pyroptosis in decidua tissue.Pearson method was used to analyze the correlation between pyroptosis rate and the protein levels of NLRP3,IL-1β,Caspase1 and GSDMD in decidua.Results Compared with the control group,expression levels of NLRP3,IL-1β,Caspase1,GSDMD mRNA and protein,the cell pyroptosis rate in decidua tissues were significantly increased in the research group(P<0.05).There was a positive correlation between cell pyroptosis rate in decidua tissue and expression levels of NLRP3,IL-1β,Caspase1 and GSDMD in the research group(r=0.429,0.474,0.498,0.540,all P<0.05).Conclusion The expression levels of pyrolysis-related factors NLRP3,IL-1β,Caspase1 and GSDMD in decidua tissue are increased in patients with early abortion after IVF/ICSI assisted pregnancy,and which are related to the cell pyroptosis of decidua tissue.