黄芩素联合nisin对金黄色葡萄球菌生物被膜的体外作用

In vitro study on the effect of baicalein combined with nisin on biofilm of Staphylococcus aureus

金黄色葡萄球菌的生物被膜是引起其增殖扩散并产生毒素的重要原因。该文探究乳酸链球菌素(nisin)与黄芩素联合使用对金黄色葡萄球菌生物被膜的抑制效果。通过筛选确定nisin与中药成分联用对生物被膜的抑制效果及最佳浓度配比,并初步探究二者联用对金黄色葡萄球菌生物被膜的抑制机制。结果表明nisin与黄芩素对金黄色葡萄球菌生物被膜的MIC_(50)分别为0.5、0.1 mg/mL;二者联用时多个浓度配比对生物被膜均有协同抑制作用,同时可以降低剂量,其中最佳质量浓度配比为0.0625 mg/mL的nisin和3.125μg/mL的黄芩素;二者联用可降低生物被膜的黏附性、疏水作用、自动聚集能力、胞外多糖和eDNA的产生,表明其可抑制生物被膜的黏附、生长和成熟;在18 h时nisin与黄芩素对生物被膜的抑制和清除效果最佳;通过扫描电镜观察到生物被膜附着大量减少,菌落分明。nisin与黄芩素联用可协同抑制金黄色葡萄球菌生物被膜的形成。

The biofilm of Staphylococcus aureus is an important reason for its proliferation and toxin production.This paper explores the combined use of nisin and baicalein to enhance the inhibitory effect on S.aureus biofilm.It’s also screened and determined the inhibitory effect and optimal concentration ratio of nisin combined with traditional Chinese medicine ingredients on biofilm,and explored the mechanism of action in inhibiting biofilm formation.The results showed that the MIC_(50) values of nisin and baicalein were 0.5 and 0.1 mg/mL,respectively.These multiple concentration ratios have synergistic inhibitory effect on biofilm and obtain content anti-biofilm effect with lower dose.The optimal concentration combination was 0.0625 mg/mL nisin and 3.125μg/mL baicalein.This combination also reduced the adhesion,hydrophobic effect,autofocusing ability,extracellular polysaccharide,and eDNA production of biofilm,indicating that they could inhibit the adhesion,growth,and maturation of biofilm.At 18 h,the inhibition and scavenging effect on biofilm was better than others.Scanning electron microscope results showed that the biofilm attachment was greatly reduced and the colonies were distinct.In conclusion,the combination of nisin and baicalein can synergistically inhibit the formation of S.aureus biofilm,further establish the foundation for the development of more efficient natural food preservative.