鸡血藤提取物对α-淀粉酶和α-葡萄糖苷酶的抑制作用

α-Amylase andα-glycosidase inhibitory activities of the extracts from Spatholobus suberectus

从鸡血藤中获得醇提物(alcohol extract,ESs)及水提物(water extracts,WSs),研究其对α-淀粉酶和α-葡萄糖苷酶的抑制作用。结果表明,ESs的抑制效果显著高于WSs。为深入研究鸡血藤对2种酶的抑制作用,采用不同极性溶剂对ESs进一步萃取,获得W-ESs、D-ESs、N-ESs、E-ESs四个组分。实验结果表明,在同一浓度范围,ESs及4个组分的抑制效果存在差异。对α-淀粉酶的抑制效果为:W-ESs>D-ESs>ESs>N-ESs>E-ESs;对α-葡萄糖苷酶的抑制效果为:W-ESs>ESs>N-ESs>E-ESs>D-ESs。其中W-ESs对2种酶的抑制作用最好,IC_(50)值分别为(0.88±0.02)mg/mL和(15.82±2.79)μg/mL。绘制Lineweaver-Burk曲线确定酶抑制类型,结果显示,WSs、ESs、W-ESs及N-ESs对α-淀粉酶为反竞争性抑制,D-ESs和E-ESs为竞争性和非竞争性混合抑制。对于α-葡萄糖苷酶,W-ESs、D-ESs和E-ESs为反竞争性抑制类型,而WSs、ESs和N-ESs为反竞争性和非竞争性混合的抑制类型。研究结果表明,鸡血藤具有开发为降血糖药物的潜力。

In this study,ethanol extracts(ESs)and water extracts(WSs)were obtained from Spatholobus suberectus.The inhibition ofα-amylase andα-glucosidase were determined.The results showed that the inhibition effect of ESs was significantly higher than that of WSs.To further explore the inhibition of the two enzymes by S.suberectus,the ESs was further separated by different polar solvents and obtained four components:W-ESs,D-ESs,E-ESs,and N-ESs.The different inhibitory effects of ESs and the four components at the same concentration range were as follows,α-amylase:W-ESs>D-ESs>ESs>N-ESs>E-ESs;α-glucosidase:W-ESs>ESs>N-ESs>E-ESs>D-ESs.W-ESs showed the best inhibition effect for both enzymes,with IC_(50) values of(0.88±0.02)mg/mL and(15.82±2.79)μg/mL.The Lineweaver-Burk curve was plotted to determine the type of enzyme inhibition.The results showed that WSs,ESs,W-ESs,and N-ESs were sensitive toα-amylase,which were the type of anti-competitive inhibition.D-ESs and E-ESs were competitive and non-competitive mixed inhibition.Forα-glucosidases,WEs,D-ESs and E-ESs were the type of anti-competitive inhibition,while WSs,ESs and N-ESs were competitive and non-competitive mixed inhibition type.Our results showed that S.suberectus had the potential to be developed as a hypoglycemic drug.