非小细胞肺癌中Ki-67和Bcl-2、HMGB1表达的临床意义

Clinical Significance of Ki-67,Bcl-2 and HMGB1 Expression in Non-small Cell Lung Cancer

目的:研究非小细胞肺癌(NSCLC)中增殖细胞核抗原-67(Ki-67)和B细胞淋巴瘤因子2蛋白(Bcl-2)、高迁移率族蛋白1(HMGB1)表达的临床意义。方法:选择2017年1月-2019年6月广州市中西医结合医院收治的84例NSCLC患者。采集所有患者NSCLC组织及癌旁正常组织,对比不同肺组织Ki-67、Bcl-2及HMGB1表达情况。分析Ki-67、Bcl-2及HMGB1表达与NSCLC患者临床病理特征及预后的关系。结果:NSCLC组织中Ki-67、Bcl-2及HMGB1阳性表达率分别为58.33%、45.24%、75.00%,相较于癌旁正常组织的9.52%、8.33%、13.10%更高(P<0.05)。临床分期Ⅲ、Ⅳ期和有淋巴结转移NSCLC患者中Ki-67阳性率相较于临床分期Ⅰ、Ⅱ期和无淋巴结转移患者更高(P<0.05)。有淋巴结转移和组织学分级Ⅲ级NSCLC患者的Bcl-2阳性率相较于无淋巴结转移和组织学分级Ⅰ、Ⅱ级患者更高(P<0.05)。有淋巴结转移、组织学分级Ⅲ级和临床分期Ⅲ、Ⅳ期NSCLC患者的HMGB1阳性率相较于无淋巴结转移、组织学分级Ⅰ、Ⅱ级和临床分期Ⅰ、Ⅱ期患者更高(P<0.05)。NSCLC死亡患者Ki-67、Bcl-2、HMGB1阳性率分别为85.71%、68.57%、94.29%,相较于存活组的38.78%、28.57%、61.22%均较高(P<0.05)。结论:NSCLC中Ki-67、Bcl-2及HMGB1均呈异常高表达,且均和预后密切相关。

Objective:To investigate the clinical significance of proliferating cell nuclear antigen 67(Ki-67),B-cell lymphoma factor 2 protein(Bcl-2)and high mobility group protein 1(HMGB1)expression in non-small cell lung cancer(NSCLC).Method:A total of 84 patients with NSCLC admitted to the Guangzhou Hospital of Integrated Traditional Chinese and Western Medicine from January 2017 to June 2019 were selected.NSCLC tissues and adjacent normal tissues were collected from all patients,and the expressions of Ki-67,Bcl-2 and HMGB1 in different lung tissues were compared.The relationship between Ki-67,Bcl-2 and HMGB1 expression and clinicopathologic features and prognosis of NSCLC patients were analyzed.Result:The positive expression rates of Ki-67,Bcl-2 and HMGB1 in NSCLC tissues were 58.33%,45.24%and 75.00%,which were higher than 9.52%,8.33%and 13.10%in adjacent normal tissues(P<0.05).The positive rate of Ki-67 in patients with stageⅢ,stageⅣand NSCLC with lymph node metastasis were higher than those in patients with stageⅠ,stageⅡand without lymph node metastasis(P<0.05).The positive rate of Bcl-2 in NSCLC patients with lymph node metastasis and histological gradeⅢwere higher than those in patients without lymph node metastasis and histological gradeⅠandⅡ(P<0.05).The positive rate of HMGB1 in patients with lymph node metastasis,histological gradeⅢand clinical stageⅢandⅣwere higher than those in patients without lymph node metastasis,histological gradeⅠandⅡ,and clinical stageⅠandⅡ(P<0.05).The positive rates of Ki-67,Bcl-2 and HMGB1 in dead NSCLC patients were 85.71%,68.57%and 94.29%,respectively,which was higher than 38.78%,28.57%and 61.22%in the survival group(P<0.05).Conclusion:The expressions of Ki-67,Bcl-2 and HMGB1 are abnormally high in NSCLC,which are closely related to the prognosis.