高效液相色谱-电雾式检测器法同时检测食品中8种人工甜味剂的应用

Research on Simultaneous Determination of Eight Artificial Sweeteners in Food by High Performance Liquid Chromatography with Charged Aerosol Detector

采用高效液相色谱-电雾式检测器(HPLC-CAD)建立了同时测定食品中8种人工甜味剂的方法。以5 mmol/L甲酸铵和乙腈为流动相进行梯度洗脱,CAD检测器直接检测,外标法定量。研究结果表明,安赛蜜、糖精钠、甜蜜素、三氯蔗糖、阿斯巴甜、阿力甜、爱德万甜和纽甜在相应线性范围内线性关系良好(相关系数R^(2)>0.999),检出限为5~30μg/L,定量限为20~150μg/L,3个浓度水平的加标回收率在88.5%~102.8%之间,相对标准偏差为0.85%~2.16%。该方法简单、快速、灵敏度高和重复性好,可用于不同食品中人工甜味剂的有效检测研究。

The sweeteners are separated by high performance liquid chromatography(HPLC)with 5 mmol/L ammonium formate-acetonitrile gradient elution and quantified by charged aerosol detector(CAD)to establish a method for the simultaneous determination of eight artificial sweeteners in food by HPLC-CAD.The linearity of acesulfame,sodium saccharin,cyclamate,sucralose,aspartame,alitame,advantame and neotame in the range is good,and the correlation coefficient is above 0.999.The limit of detection and quantitation is 5~30μg/L and 20~150μg/L,respectively.The recovery rates are 88.5%to 102.8%,and the relative standard deviations are less than 2.16%.The method is simple,rapid,sensitive and reproducible,and can be used for the detection of 8 sweeteners in food.