不同杂交组合白叶枯病抗性鉴定及遗传多样性分析

Identification of Bacterial Blight Resistance and Analysis of Genetic Diversity in Different Cross Combinations

为提高抗白叶枯病新品种选育效率,减少育种的盲目性,本实验以87份杂交水稻品种为研究对象进行水稻白叶枯病鉴定,同时在基于SSR分子标记技术上进行遗传多样性分析。通过白叶枯病表型聚类和SSR分子标记聚类相结合方式进行分析,选择在抗白叶枯病方面具有优势的亲本。研究结果表明:在以优良不育系望S、粤禾丝苗、Y58S和优良恢复系R301、N111S为亲本的杂交水稻的白叶枯病的等级较低。在遗传多样性分析方面,筛选出36对具有多态性位点的SSR引物,分子量范围为90~200 bp;共扩增出118个等位基因,87份杂交稻品种在不同位点等位基因数目平均为3.3个,范围为2~6个,平均Nei’s基因多样性指数为0.452,变幅为0.096(SSR03)~0.764(SSR19)。亲本不育系望S、粤禾丝苗、Y58S和恢复系R301、N111S在选育抗白叶枯新品种时具有一定育种价值,可以为育种家选育抗白叶枯病新品种提供参考。

In order to improve the efficiency of breeding new varieties resistant to bacterial blight and reduce the blindness of breeding,in this study,87 hybrid rice varieties were identified for bacterial blight,and genetic diversity was analyzed based on SSR molecular markers.By combining phenotypic clustering with SSR molecular marker clustering,the parents with advantages in resistance to bacterial blight were selected.The results showed that the grade of bacterial blight of hybrid rice with good sterile lines WangS,Yuehe simiao,Y58S and good restorer lines R301 and N111S is low.In terms of genetic diversity analysis,36 pairs of SSR primers with polymorphic loci were screened,with molecular weights ranging from 90~200 bp.A total of 118 alleles were amplified,and the average number of alleles at different loci of 87 hybrid rice varieties is 3.3,ranging from 2 to 6.The average Nei's gene diversity index is 0.452,with a range of 0.096(SSR03)to 0.764(SSR19).The parental sterile lines WangS,Yuehe simiao,Y58S and the restorers R301 and N111S have certain breeding value in breeding new varieties resistant to bacterial blight,which can provide reference for breeders to select new varieties resistant to bacterial blight.