高效脱氮降磷菌株的筛选及在工厂化鳗鲡养殖中的应用

Screening of a high-efficient nitrogen and phosphorus removal functional strain and application research in eel intensive culture

为提高对工厂化鳗鲡精养殖水体水质的原位改良能力和养殖尾水的氮磷减排,通过对鳗鲡养殖尾水处理池中采集的淤泥进行分离筛选,获得一株高效好氧反硝化聚磷菌。经16S rDNA序列鉴定分析,确定为坦氏不动杆菌(Acinetobacter tandoii),将其命名为坦氏不动杆菌PP-1。开展了菌株PP-1对鳗鲡养殖水质总磷和硝酸盐氮降解效果的试验,结果显示水力停留时间在12 h、24 h的总磷降解率分别达到56.31%和81.82%,硝酸盐氮降解率分别达到65.5%和90.37%。开展了菌株PP-1在美洲鳗鲡工厂化养殖中的应用研究,结果显示,水力停留时间在24 h的总磷和硝酸盐氮的降解率分别达到58.8%和56.1%。研究表明,坦氏不动杆菌PP-1有助于降低美洲鳗鲡养殖水体的总磷和硝酸盐氮质量浓度,促进美洲鳗鲡的生长,同时具备良好的应用安全性。该研究为解决鳗鲡养殖水体中的氮磷同步降解问题提供了新的参考,具有重要的应用价值。

To improve the water quality of eel intensive culture and the nitrogen and phosphorus reduction in aquaculture tailwater,this research was conducted to isolate and screen the sludge collected from the treatment pond of eel culture tailwater and got a strain of high-efficient aerobic denitrifying phosphorus removing bacteria.It was identified as Acinetobacter tandoii by 16S rDNA sequence identification analysis and named Acinetobacter tandoii PP-1.An experiment on the total phosphorus and nitrate degradation effect of strain PP-1 in the water quality of the Anguilla culture was carried out.The results showed that the total phosphorus degradation rate reached 56.31%and 81.82%at 12 h and 24 h hydraulic retention time,respectively.And the nitrate nitrogen degradation rate reached 65.5%and 90.37%at 12 h and 24 h hydraulic retention time,respectively.Application research of strain PP-1 in water quality control of Anguilla rostrata factory farming showed that the degradation rate of total phosphorus and nitrate nitrogen with hydraulic residence time at 24 h reached 58.8%and 56.1%,respectively.This study showed that A.tandoii PP-1 could help improve the total phosphorus and nitrate nitrogen in the culture water of Anguilla rostrata,and promote the growth of Anguilla rostrata,with good application safety.This study provides a new reference for solving the problem of simultaneous degradation of nitrogen and phosphorus in eel culture water and has important application value.