ARID5B基因SNP与ALL患儿MTX耐药性的相关性

Correlation between ARID5B Gene SNP and MTX Resistance in Children with ALL

目的:探讨ARID5B基因单核苷酸多态性(SNP)与急性淋巴细胞白血病(ALL)患儿甲氨蝶呤(MTX)耐药性的相关性。方法:纳入2015年1月至2021年11月于宁夏医科大学总院治疗的144例ALL患儿,分为MTX耐药组和非MTX耐药组,每组72例,采用基质辅助激光解吸电离飞行时间质谱技术测量所有患儿的ARID5B基因SNP,分析其与患儿MTX耐药性的相关性。结果:两组患儿rs7923074、rs10821936、rs6479778、rs2893881位点基因型及基因频率比较无统计学差异(P>0.05);MTX耐药组C/C基因型频率明显高于非MTX耐药组,而T/T基因型频率则相反(P<0.05);MTX耐药组C等位基因频率明显高于非MTX耐药组,而T等位基因频率则相反(P<0.05);多因素Logistic回归分析结果显示,ARID5B基因rs4948488 TT基因型、T等位基因频率是ALL患儿MTX耐药性的危险因素(P<0.05)。结论:ARID5B基因SNP与ALL患儿MTX耐药性具相关性。

Objective:To investigate the correlation between single-nucleotide polymorphism(SNP)of ARID5B gene and resistance to methotrexate(MTX)in children with acute lymphoblastic leukemia(ALL).Methods:A total of 144 children with ALL who were treated in General Hospital of Ningxia Medical University from January 2015 to November 2021 were enrolled and divided into MTX resistant group and non-MTX resistant group,with 72 cases in each group.Matrix-assisted laser desorption/ionization time of flight mass spectrometry(MALDI-TOF MS)technology was used to measure the SNP of ARID5B gene in all children and analyze its correlation with MTX resistant.Results:There were no significant differences in the genotype and gene frequency of rs7923074,rs10821936,rs6479778,and rs2893881 between MTX resistant group and non-MTX resistant group(P>0.05).The frequency of C/C genotype in the MTX resistant group was significantly higher than that in the non-MTX resistant group,while the frequency of T/T genotype was opposite(P<0.05).The frequency of C allele in the MTX resistant group was significantly higher than that in the non-MTX resistant group,while the frequency of T allele was opposite(P<0.05).Multivariate logistic regression analysis showed that ARID5B gene rs4948488 TT genotype and T allele frequency were risk factors for MTX resistant in ALL children(P<0.05).Conclusion:The SNP of ARID5B gene is associated with MTX resistant in ALL children.