栓菌4281漆酶催化几种不同结构染料脱色

Decolorization of several different dyes by Trametes sp.4281 laccase

栓菌4281菌株是实验室自主筛选鉴定的新型漆酶高效生产菌株,为探究其漆酶对染料脱色的作用效果,利用栓菌4281漆酶粗酶液对偶氮、蒽醌和苯甲烷类3种类型的染料进行脱色反应,分别考察不同介体类型、温度、pH值、介体浓度、给酶量、反应时间以及金属离子对染料脱色的影响。结果表明:添加介体对染料脱色效率有显著的促进作用,6种染料在8 h内基本反应完全,在各染料最适反应条件下活性蓝、茜素红、甲基橙和结晶紫的最大脱色率分别达到93.9%、92.3%、90%和90%,刚果红和碱性品红脱色率达到76.6%和75%;Mn^(2+)和高浓度的Fe^(2+)对栓菌4281漆酶催化染料脱色作用有一定的抑制,而在Na+、Mg^(2+)、Cu^(2+)、Ni^(2+)和Zn^(2+)金属离子存在下该漆酶仍能对染料保持较高的脱色率。研究表明:栓菌4281漆酶在染料脱色中具有较大的应用潜力;在环境废水的治理中具有良好的应用前景。

Trametes sp.4281 is a new type of highly efficient laccase production strain independently screened and identified in the laboratory.In order to explore the potential of laccase to decolorize dye,crude Trametes sp.4281 laccase solution was used to decolorize three categories of dyes,i.e.azo,anthraquinone and phenylmethane.The effects of different mediums,temperatures,pHs,medium concentration,enzyme amount,reaction times and metal ions on the dye decolorization were investigated.The results showed that addition of mediators had a significant influence on the decolorization efficiency;the six dyes were almost completely decolorized within 8 h,with the decolorization rates of reactive blue,alizarin red,methyl orange and crystal violet under the optimum reaction conditions reaching 93.9%,92.3%,90%and 90%,and those of Congo red,fuchsin reaching 76.6%and 75%,respectively.Mn^(2+)and high concentrations of Fe^(2+)had inhibitory effect on the decolorization,while in the presence of metal ions of Na+,Mg^(2+),Cu^(2+)Ni^(2+),and Zn^(2+)the laccase remained high decolorization rates to the dyes.The results suggest that Trametes sp.4281 laccase has great application potential in the dye decolorization,and has a good prospect in the treatment of environmental wastewater.