菊花提取物对金黄色葡萄球菌的抑菌作用研究

Antibacterial Effect of Chrysanthemum Extract on Staphylococcus aureus

采用水煎煮法对菊花中的抑菌物质进行提取,以抑菌圈大小为指标测定提取物对金黄色葡萄球菌的抑菌活性,并采用单因素试验及正交试验对菊花提取物的提取工艺进行优化。结果表明,菊花水提物对金黄色葡萄球菌有一定的抑制作用,具有最强抑菌作用的菊花水提物的提取条件为料液比1∶40,提取温度100℃,提取时间1.5 h,在此条件下的抑菌圈直径达到最大值为0.96 cm。

The antibacterial substances in chrysanthemum were extracted by water decoction method,and the antibacterial activity of the extract against Staphylococcus aureus was determined by taking the size of antibacterial circle as the index.The extraction process of chrysanthemum extract was optimized by single factor test and orthogonal test.The results showed that the aqueous extract of chrysanthemum had significant inhibitory effect on Staphylococcus aureus.The extraction conditions of the aqueous extract of chrysanthemum with the strongest antibacterial effect were that the material liquid ratio was 1∶40,the extraction temperature was 100℃and the extraction time was 1.5 h.Under this condition,the maximum diameter of bacteriostatic circle was 0.96 cm.