黄连须多糖降血糖活性及结构表征

Hypoglycemic activity and structure characterization of Coptis Rhizoma fibrous polysaccharides

目的探究黄连副产品黄连须多糖(Coptidis Radix fibrous polysaccharides,CRFP)的降血糖活性和物质基础。方法研究CRFP对糖尿病模型小鼠的作用,并对其进行分离纯化,检测纯化产物的纯度和相对分子质量,分析其结构和单糖组成。结果给药8周后,CRFP能显著降低糖尿病模型小鼠的血糖(P<0.01),加速小鼠的血糖和血脂代谢,提高空腹胰岛素水平(P<0.01),改善血脂紊乱(P<0.05、0.01),提高肝脏己糖激酶(hexokinase,HK)和丙酮酸激酶(pyruvate kinase,PK)活性(P<0.01),改善氧化应激(P<0.05、0.01)。CRFP经纯化后得到成分均一的多糖CRFP-1和CRFP-2,相对分子质量分别为24307±311和18405±168,CRFP-1为α-型多糖,由8种单糖组成;CRFP-2为β-型多糖,由10种单糖组成。结论CRFP具有良好的降血糖活性,为黄连这一传统中药的深度开发和提高利用价值奠定了基础。

Objective To explore the hypoglycemic activity and material basis of Huanglian(Coptis Rhizoma)fibrous polysaccharides(CRFP),a by-product of Coptis chinensis.Methods The effect of CRFP on diabetes model mice was studied and CRFP was purified.The purity and relative molecular weight of purified product were detected,and its structure and monosaccharide composition were analyzed.Results After eight weeks of administration,CRFP could significantly reduce blood glucose(P<0.01),accelerate blood glucose and lipid metabolism,increase fasting insulin level(P<0.01),improve blood lipid disorder(P<0.05,0.01),increase liver hexokinase(HK)and pyruvate kinase(PK)activities(P<0.01),and improve oxidative stress(P<0.05,0.01)in diabetes model mice.After purification,CRFP-1 and CRFP-2 with uniform components were obtained.The relative molecular weight of CRFP-1 and CRFP-2 respectively were(24307±311)and(18405±168),CRFP-1 wasα-type polysaccharide,composed of eight monosaccharides;CRFP-2 wasβ-type polysaccharide,composed of 10 monosaccharides.Conclusion CRFP has good hypoglycemic activity,which lays a foundation for the deep development and improvement of utilization value of C.chinensis.