HPLC测定壮药羊开口中3,3′,4′-三甲氧基鞣花酸-4-O-β-D-吡喃葡萄糖苷的含量

Determination of 4-O-β-D-glucopyranosyl-3,3',4'-tri-O-methylellagic acid in Zhuang Medicine Radix Melastomatis Normalis by HPLC

目的建立HPLC测定壮药羊开口中3,3',4'-三甲氧基鞣花酸-4-O-β-D-吡喃葡萄糖苷的含量方法。方法采用Welch Materials Ultimate AQ C_(18)反相色谱柱(250 mm×4.6 mm,5μm);流动相为乙腈-0.2%磷酸水溶液,采用梯度洗脱,体积流量为1.0 mL/min,检测波长为246 nm,柱温为35℃。结果3,3',4'-三甲氧基鞣花酸-4-O-β-D-吡喃葡萄糖苷进样量在0.02~0.60μg内与峰面积线性关系良好(r=1.0000);平均回收率为105.58%,RSD为2.27%。结论该方法简便、重复性好、准确度高,可用于壮药羊开口的质量控制。

Objective To establish a HPLC method for the determination of 4-O-β-D-glucopyranosyl-3,3',4'-tri-O-methylellagic acid in Zhuang Medicine Radix Melastomatis normalis.Methods A Welch Materials Ultimate AQ C_(18) reverse phase column(250 mm×4.6 mm,5μm)was used.The mobile phase consisted of acetonitrile-water containing 0.2%phosphoric acid solution with gradient elution at a flow rate of 1.0 mL/min.The detection wavelength was set at 246 nm and the temperature of column was 35℃.Results The linear relationship between 4-O-β-D-glucopyranosyl-3,3',4'-tri-O-methylellagic acid injection amount and peak area was good in the range of 0.02—0.60μg(r=1.0000).The average recovery was 105.58%with RSD of 2.27%.Conclusion The method is simple,reproducible and accurate,and can be used for the quality control of Zhuang Medicine Radix Melastomatis normalis.