牡丹促成栽培中糖信号调控成花的机理研究

The Mechanism of Sugar Signal Involved in Regulating Re-flowering of Tree Peony Under Forcing Culture

以牡丹栽培品种‘洛阳红’为试材,在促成栽培条件下对花芽进行剥叶和涂抹赤霉素处理,探究其对花芽发育、叶片气孔特征、葡萄糖、果糖、蔗糖和海藻糖-6-磷酸(T6P)水平以及糖信号相关基因表达模式等的影响。结果表明,剥叶和赤霉素处理均有效促进牡丹花蕾发育且能够显著促进叶片气孔的发育,提高气孔的开度;剥叶和赤霉素处理能够促进蔗糖和葡萄糖的代谢,并通过促进T6P的积累触发糖信号,最终通过上调PsTPS1及抑制PsSnRK1和PsHXK1在叶片中的表达,诱导花蕾发育。

In this study,tree peony cultivar‘Luoyang Hong’was used as the material.Under the conditions of forcing culture,the flower buds were defoliated and treated with gibberellin to explore the effects on the development of flower buds,leaf stomatal characteristics,and glucose,fructose,sucrose,trehalose-6-phosphate(T6P)levels and the expression patterns of genes related to sugar signaling pathway.The results showed that both defoliation and gibberellin treatments can effectively promote the development of flower buds and can significantly promote the development of stomata and increase the opening of the stomata;at the same time,defoliation and gibberellin treatments could promote the metabolic consumption of energy substances,such as sucrose and glucose.The sugar signaling pathway was triggered by sensing the accumulation of sugar signaling molecule T6P content,and finally it promoted the development of flower bud by up-regulating the expression of PsTPS1 in leaves and inhibiting the expression of PsSnRK1 and PsHXK1.