基于SSR标记的42份樱花品种的聚类分析及DNA指纹图谱构建

Cluster Analysis and Construction of DNA Fingerprinting of 42 Oriental Cultivars of Flowering Cherry Based on SSR Markers

通过14对SSR引物基于UPGMA法对42份樱花品种进行聚类分析,并采用引物和基因型组合法构建DNA指纹图谱。结果表明:14对SSR引物共检测出187个等位基因,每对引物平均13.36个,有效等位基因平均6.10个,观察杂合度平均0.31,期望杂合度平均0.80,Shannon’s信息指数平均2.01,多态信息含量平均0.77;共检测出219个基因型,平均15.64个,平均区分率为22.11%。在Dice遗传相似系数约为0.18处时可分为3组,A组主要是山樱系、豆樱系和江户彼岸樱系的品种,B组是钟花樱系的品种,C组中只有尾叶樱;当Dice遗传相似系数约为0.37时,除八重红枝垂樱外,A组又可以分为6个小组。采用引物和基因型组合法构建了42份樱花品种的DNA指纹图谱,为今后樱花品种资源的分子鉴定提供依据。

In this study,14 pairs of SSR primers were used to cluster 42 oriental cultivars of flowering cherry based on UPGMA method,and to construct DNA fingerprints by combining primers and genotypes.The results showed that a total of 187 alleles were detected from 14 pairs of SSR primers,with an average of 13.36 alleles per pair of primers and an average of 6.10 effective alleles.The average observed heterozygosity was 0.31,the average expected heterozygosity was 0.80,the average Shannon’s information index was 2.01,and the average polymorphism information content was 0.77.In total,there were 219 genotypes detected,with an average of 15.64 genotypes and an average identified rate of 22.11%.When the Dice genetic similarity coefficient was about 0.18,they could be divided into three groups.Group A mainly consisted of cultivars of Prunus serrulata series,P.incisa series and P.spachiana series,group B consisted of cultivars of P.campanulata series,and group C only had P.dielsiana.When the Dice genetic similarity coefficient was about 0.37,group A could be divided into six groups in addition to P.spachiana‘Plena Rosea’.Meanwhile,the DNA fingerprints of 42 oriental cultivars of flowering cherry were constructed by primers and genotypes.This study can provide a reliable basis for molecular identification of oriental cultivars of flowering cherry in the future.