不同品种葡萄皮上细菌的多样性分析

Diversity analysis of bacteria on the surface of different grape varieties

以4种市面常见葡萄品种:黑提(H)、玫瑰香(M)、阳光玫瑰(Y)、红提(D)为研究对象,应用高通量测序技术对4种葡萄果皮上细菌的多样性进行分析研究.试验结果表明,细菌门水平上的优势菌群一般是:拟杆菌门(Bacteroidetes)、厚壁菌门(Firmicutes)、变形菌门(Proteobacteria)、放线菌门(Actinobacteria)这4个门类,细菌属水平的优势菌群一般为乳酸菌(Lactobacillus)、拟球菌(Bacteroides)、另枝菌属(Alistipes)、毛螺旋菌科_NK4A136_group(Lachnospiraceae_NK4A136_group)、链球菌(Streptococcus)、拟普雷沃菌属(Alloprevotella).研究表明:4种葡萄表皮的细菌多样性指数无显著差异,其中玫瑰香与红提的细菌群落相似程度较高.

Taking four common grape varieties in the market as the research objects,namely,black grape,muscat grape,shine-muscat and red grape,the diversity of bacteria on the surface of four grape varieties was analyzed by high-throughput sequencing technology.The experimental results show that the dominant flora at the level of bacteria phylum mainly includes four categories:Bacteroidetes,Firmicutes,Proteobacteria and actinomycetes.At the level of bacteria genus,the dominant flora are mainly Lactobacillus,Bacteroides,Alistipes,lachnospiraceae NK4A136_group,Streptococcus and Alloprevotella.The results show that there is no significant difference in the bacterial diversity index of the surface of the four grapes,among which the bacterial communities of muscat grape and red grape are more similar.