不同种群白纹伊蚊单倍型多态性、遗传分化及种系发育关系分析

Haplotypes Diversity,Genetic Differentiation and Phylogenetic Analysis of Aedes albopictus

【目的】试验旨在探究湖南怀化地区不同种群白纹伊蚊单倍型多态性、遗传分化情况及种系发育关系。【方法】于湖南怀化地区采集30只白纹伊蚊样本,PCR扩增其核糖体18S rRNA及线粒体细胞色素c氧化酶亚基Ⅰ(cox1)基因部分序列。利用ClustalX软件对其变异位点进行分析;运用Dnasp 5.0、Network 4.6及Arlequin version 3.0软件进行单倍型多态性与遗传分化分析;利用PhyML 3.0及Mega 5.0软件对白纹伊蚊的种系发育关系进行分析。【结果】所获全部样本的18S rRNA和cox 1基因序列长度均为489和1004 bp。白纹伊蚊18S rRNA基因序列中共存在30个变异位点,17个单倍型(A1~A17,Hd=0.9149),其中单倍型A5为最古老的单倍型,白纹伊蚊18S rRNA的基因分化系数(Gst)、遗传分化系数(Fst)和基因流(Nm)分别为0.05614、0.24001和0.792;白纹伊蚊的cox 1基因序列中共存在2个变异位点,3个单倍型(B1~B3,Hd=0.1908),其中单倍型B1为最古老的单倍型,白纹伊蚊cox 1基因的Gst、Fst和Nm分别为0.05000、0.04762和4.999。系统发育树中,来源于怀化地区的30只白纹伊蚊均分别处于2个发育树的一个分支。【结论】来源于湖南怀化地区的白纹伊蚊个体和种群之间存在一定程度基因变异和单倍型多态性现象,但各单倍型及其个体之间具有较近的亲缘关系,且两种群白纹伊蚊个体间发生了较为频繁的基因交流,未出现遗传分化。

【Objective】The purpose of this study was to explore the haplotypes diversity,genetic differentiation and phylogenetic relationships of Aedes albopictus in different populations in Huaihua district,Hunan province.【Method】Thirty Aedes albopictus were collected from Huaihua district,Hunan province.The partial sequences of the ribosomal 18S rRNA and cytochrome c oxidase subunit 1(cox1)gene from Aedes albopictus were amplified using PCR method.The variation sites on both genes sequences were analyzed with ClustalX and the haplotypes diversity,genetic differentiation and phylogenetic relationships of Aedes albopictus were studied by Dnasp 5.0,Network 4.6,Arlequin version 3.0,PhyML 3.0 and Mega 5.0,respectively.【Result】The results showed that the 18S rRNA and cox 1 gene sequences of all samples was 489 and 1004 bp,respcetively.A total of 30 mutation loci were found in the 18S rRNA sequences of Aedes albopictus,including 17 haplotypes(A1 to A17,Hd=0.9149),among which haplotype A5 was the oldest haplotype.The gene differentiation coefficient(Gst),genetic differentiation coefficient(Fst)and gene flow(Nm)of 18S rRNA gene of Aedes albopictus were 0.05614,0.24001 and 0.792,respectively.There were 2 mutation loci and 3 haplotypes(B1 to b3,Hd=0.1908)in the cox 1 gene sequence of Aedes albopictus.Haplotype B1 was the oldest haplotype.The Gst,Fst and Nm of cox 1 gene of Aedes albopictus were 0.05000,0.04762 and 4.999,respectively.Among the phylogenetic tree,30 Aedes albopictus from Huaihua were located in a branch of two developmental trees.【Conclusion】The results indicated that there was a certain degree of genetic variation and haplotype polymorphism among the individuals and populations of Aedes albopictus from Huaihua district,Hunan province.However,the haplotypes and individuals had the close genetic relationships,and the frequent genetic exchanges occurred between the individuals of the two populations,whithout genetic differentiation.