四逆汤加减对仔猪腹泻治疗效果研究

Study on the Therapeutic Effect of Modified Sini Decoction on Piglet Diarrhea

【目的】探究四逆汤加减对仔猪腹泻的治疗效果及生长性能、血液生理生化指标和肠道菌群的影响。【方法】选取体重相近的3日龄腹泻仔猪120头,随机分为中药组和西药组,每组60头,另选同群健康仔猪60头为空白对照组。中药组每头仔猪每天灌喂5 g四逆汤加减中药液,连喂7 d;西药组按0.1 mg/kg BW肌内注射庆大霉素,每天1次,连用4 d;空白对照组不做药物处理。给药期间观察并记录仔猪精神状态、采食情况、腹泻情况、平均日增重;给药前后,分别测定仔猪血液生理生化指标及肠道微生物菌群。【结果】用药5 d后,中药组治愈率达97%,显著高于西药组(87%)(P<0.05),且未出现死亡。治疗结束后,中药组仔猪平均日增重显著高于西药组和空白对照组(P<0.05)。血常规结果显示,中药组白细胞(WBC)、淋巴细胞(LYM)、单核细胞(MONO)、中性粒细胞(GRAN)数量及红细胞压积(HCT)均显著高于西药组(P<0.05);其他血常规指标在各组间均差异不显著(P>0.05)。血液生化结果显示,中药组丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)活性均显著低于西药组(P<0.05),肌酐(CRE)含量显著高于西药组(P<0.05),各血液生化指标与空白对照组均差异不显著(P>0.05)。肠道菌群高通量测序结果显示,中药组肠道菌群Simpson、Shannon指数均显著高于西药组(P<0.05);与西药组相比,中药处理能提高厚壁菌门和拟杆菌门丰度,降低变形杆菌门和梭杆菌门丰度;增加乳杆菌属和梭菌属细菌,减少大肠杆菌属、芽孢杆菌属、肠球菌属细菌。【结论】四逆汤加减能有效治疗仔猪腹泻,且效果优于西药组,并能有效提高腹泻仔猪生长性能,促进机体损伤的修复,调节肠道菌群结构。

【Objective】This study was aimed to explore the therapeutic effect of Sini decoction on piglet diarrhea and the influence of growth performance,blood physiological and biochemical indicators and intestinal flora.【Method】One hundred and twenty 3-day-old diarrhea piglets with similar body weight were randomly divided into Chinese medicine group and Western medicine group,with 60 piglets in each group,and 60 healthy piglets of the same group were seleted as blank control group.The piglets in Chinese medicine group were fed with 5 g modified Sini decoction compound Chinese medicine solution every day for 7 days,while those in Western medicine group were injected intramuscularly with 0.1 mg/kg BW of gentamicin once a day for 4 days,blank control group was not treated with drugs.The mental status,food intake,diarrhea and average daily weight gain were recorded during the treatment period,and the blood physiological and biochemical indexes and intestinal microflora were determined before and after treatment.【Result】After 5 days of treatment,the cure rate in traditional Chinese medicine group was 97%,which was significantly higher than that in Western medicine group(87%)(P<0.05),and no death occurred.After the treatment,the average daily gain of piglets in traditional Chinese medicine group was significantly higher than that in Western medicine and blank control groups(P<0.05).The results of blood routine test showed that the amount of white blood cell(WBC),lymphocyte(LYM),mononuclear cell(MONO),neutrophil(GRAN)and hematocrit(HCT)in traditional Chinese medicine group were significantly higher than those in Western medicine group(P<0.05).There was no significant difference in other blood routine indicators between groups(P>0.05).The blood biochemical results showed that the activities of alanine aminotransferase(ALT)and aspartate aminotransferase(AST)in traditional Chinese medicine group were significantly lower than those in Western medicine group(P<0.05),the creatinine(CRE)content was significantly higher than that of Western medicine group(P<0.05),there was no significant difference between traditional Chinese medicine and blank control groups(P>0.05).High-throughput sequencing of intestinal flora showed that,compared with Western medicine group,the Simpson and Shannon indexes of intestinal flora in traditional Chinese medicine group were significantly higher(P<0.05),and the abundance of Firmicutes and Bacteroides,Lactobacillus and Clostridium were increased,the abundance of Proteobacteria and Fusobacteria,E.coli,Bacillus and Enterococcus were decreased.【Conclusion】Modified Sini decoction could effectively treat diarrhea piglets,and the effect was better than Western medicine group,and could effectively improve the growth performance of diarrhea piglets,promote the body damage repair,adjust the structure of intestinal flora.