首发抑郁症患者肠道菌群多样性与抑郁症状的相关性分析

Correlation analysis of intestinal flora diversity and clinical symptoms in patients with first-episode depression

【背景】脑-肠轴理论提示着肠道菌群和抑郁症之间存在着潜在的联系,但目前的研究争议较多,具体联系尚无定论。【目的】研究首发抑郁症患者肠道菌群多样性与抑郁症状的相关性,分析肠道菌群多样性及脱硫弧菌等菌群与抑郁症之间的潜在关系,为后续的纵向研究提供一定的理论支持。【方法】对抑郁组(n=23)和对照组(n=31)粪便中16S rRNA基因V4-V5区域片段进行测序,使用汉密尔顿抑郁量表对两组研究对象进行评估。使用α多样性检验、β多样性检验、t检验、皮尔逊相关性检验及斯皮尔曼相关性检验进行统计分析。【结果】抑郁组与对照组的肠道菌群多样性无明显差异(P>0.05)。两组肠道菌群在结构组成上有差异,属水平和种水平上,分别有28个菌属和40个菌种的相对丰度呈显著性差异(P<0.05)。抑郁组肠道菌群多样性(Shannon、Chao1指数)与抑郁症状间存在显著的正相关(P<0.05)。属水平和种水平上菌群相对丰度和抑郁症状的相关性检验中,脱硫弧菌等与抑郁症状存在显著相关性(P<0.05)。【结论】首发抑郁症患者与健康人群肠道菌群在菌群结构组成上存在差异。抑郁组肠道菌群多样性与抑郁症状存在显著的相关性,其中脱硫弧菌等菌群相对丰度和抑郁症状间存在明显的正相关。

[Background]The brain-gut axis theory suggests that there is a potential link between intestinal flora and depression,but the current research is controversial,and the specific link is not yet determined.[Objective]To study the correlation between intestinal flora diversity and depressive symptoms in patients with first-episode depression,and to analyze the potential relationship between intestinal flora diversity and Desulfovibrio and depression,thereby providing theoretical support for subsequent longitudinal studies.[Methods]The V4-V5 region fragments in the 16S rRNA gene from the feces of the depression group(n=23)and the normal group(n=31)were genetically sequenced,and the Hamilton Depression Scale was used to evaluate the two groups.Theαdiversity test,βdiversity test,t-test,Pearson correlation test,and Spearman correlation test were used for statistical analysis.[Results]There was no significant difference in the diversity of intestinal flora between the depression group and the control group(P>0.05).There were differences in the structure of intestinal flora between the two groups.The relative abundances of 28 genera and 40 species were significantly different at genus and species levels(P<0.05).There was a significant positive correlation between intestinal flora diversity(Shannon index and Chao1 index)and depressive symptoms(P<0.05).In the correlation test of the relative abundances and depressive symptoms at genus and species levels,Desulfovibrio was significantly correlated with depressive symptoms(P<0.05).[Conclusion]In this study,there are differences existed in the structure and composition of intestinal microbiota between patients with first-episode depression and healthy people.There is a significant correlation between intestinal flora diversity and depressive symptoms,and there is a significant positive correlation between the relative abundances of some flora such as Desulfovibrio and the depressive symptoms.