白桦BpAMT基因家族鉴定及表达模式分析

Genome-wide Identification and Expression Analysis of BpAMT Gene Family in Betula platyphylla

[目的]鉴定白桦AMT基因家族成员,分析AMT基因的表达模式。[方法 ]本研究利用生物信息学方法鉴定了该家族成员并通过实时荧光定量技术分析其基因表达模式。[结果 ]从白桦基因组中鉴定了9个AMT家族成员,并将其分为AMT1和AMT2两个亚家族,分别命名为BpAMT1.1~1.4和BpAMT2.1~2.5;BpAMTs氨基酸残基数为384~522个,等电点为4.61~8.16,均定位于质膜与细胞器膜上;BpAMT基因不均匀的分布在5条染色体上,且成员间存在串联重复现象。BpAMT基因的表达具有组织部位特异性,呈现叶>根>茎趋势;同时发现,硝酸钾、氯化铵、茉莉酸甲酯、赤霉素、脱落酸、氯化镉、干旱、4℃和日变化等均可以影响BpAMT基因表达,且不同处理下家族成员的响应程度存在差异。[结论 ]从白桦中鉴定9个BpAMT基因,聚类为两个亚家族,在调节氮素吸收转运、响应激素信号及非生物胁迫中发挥不同的作用,该研究结果为深入解析BpAMT基因在白桦生长发育和抵抗逆境中的功能奠定基础。

[Objective]To identify AMT gene family members in Betula platyphylla and analyze the expression pattern of AMT genes.[Method]Bioinformatics methods were used to identify the family genes and real-time fluorescence quantitative analysis of gene expression.[Result]9 AMT genes were identified from the B.platyphylla genome and divided into two subfamilies,AMT1 and AMT2,named BpAMT1.1-1.4 and BpAMT2.1-2.5;These BpAMT proteins contained 384-522 amino acid residues with isoelectric points ranging from 4.61 to 8.16,and all of which were located on the plasma membrane and organelle membrane;The genes of BpAMT family were distributed on 5 chromosomes unevenly,and there were tandem duplication among the members.BpAMTs’expression patterns had specificity in different tissues,showing a leaf>root>stem trend;At the same time,KNO3,NH4Cl,MeJA,GA3,ABA,CdCl2 and diurnal variation could affect the expression of BpAMT gene,and the response of members differed under different treatments.[Conclusion]9 BpAMT genes are identified and divided into two subfamilies.BpAMT gene plays an important role in regulating nitrogen uptake,transport and in response to hormone signals or abiotic stress.These results lay a foundation for further analysis of the function of BpAMT gene in growth and resistance to stress in B.platyphylla.