槐米提取物对肝纤维化模型小鼠的治疗作用

The therapeutic effect of Flos Sophora Immaturus extract on hepatic fibrosis model mice

目的建立肝纤维化小鼠模型,探究槐米提取物对肝纤维化模型小鼠的治疗作用。方法使用胆总管结扎方法诱导小鼠肝纤维化,将18只雄性昆明小鼠随机分为:正常组、模型组、槐米提取物治疗组(FSI组),每组6只。造模过程中观察小鼠健康状态;微板法检测各组小鼠血清谷草转氨酶(AST)和谷丙转氨酶(ALT)水平,可见分光光度法和TBA法检测各组小鼠血清还原型谷胱甘肽(GSH)和丙二醛(MDA)水平;将各组小鼠肝脏进行病理切片,观察AST、ALT、GSH、MDA水平。结果与正常组相比,模型组小鼠血清AST和ALT水平较正常组明显升高(t=16.28,P<0.001;t=7.076,P<0.01),MDA水平高于正常组(t=12.14,P<0.001),GSH水平下降(t=7.117,P<0.01)。FSI组小鼠使用槐米提取物治疗后,血清AST(t=3.775,P<0.05),ALT(t=2.98,P<0.05)水平较模型组下降,肝功能得到改善,MDA下降(t=3.421,P<0.05),GSH(t=4.681,P<0.05)升高至正常组水平。另外,模型组小鼠肝脏变性明显、粘连,表面粗糙,黄疸较为严重,FSI组小鼠肝脏无粘连,表面光滑,给药后黄疸现象消退。模型组肝细胞变性,坏死,炎性细胞浸润,胶原纤维增生形成假小叶,对比正常组纤维化明显。FSI组肝细胞结构较正常,相同面积下的炎性细胞个数减少,增生的胶原纤维较模型组减少,肝小叶结构破坏程度减轻。结论槐米提取物能减轻肝脏纤维化程度,其机制与其抗炎、抗氧化、改善肝功能异常有关。

Objective To establish the model of hepatic fibrosis,and explore the therapeutic effect of Flos Sophora Immaturus extract on the hepatic fibrosis model mice.Methods Hepatic fibrosis was caused by bile duct ligating.18 male Kunming mice were randomly divided into 3 groups:Control group,Model group and FSI treatment group(FSI group),with 6 mice in each group.The control group did nothing;Model group and FSI group were both constructed by common bile duct ligation.The health status of the mice were observed,and the microplate method was used to measure the aspartate transaminase(AST)and the alanine transaminase(ALT)in serum.The spectrophotometer method and TBA method were used to measure the glutathione(GSH)and malondialdehyde(MDA)respectively.Finally,the pathological sections were made to examine the levels of AST,ALT,GSH and MDA.Results Compared with the control group,the level of serum AST and ALT in the model group was significantly increased(t=16.28,P<0.001;t=7.076,P<0.01),the serum MDA was higher than that in the control group(t=12.14,P<0.001),and the GSH in serum was decreased(t=7.117,P<0.01).In FSI group,after the FSI extract treatment,the level of serum AST(t=3.775,P<0.05)and ALT(t=2.980,P<0.05)was significantly lower than that in the model group.As the result,the level of hepatic function improved,the level of MDA in serum was significantly lower than that in the model group(t=3.421,P<0.05),and the level of serum GSH(t=4.681,P<0.01)improved to normal.In addition,the mice in the model group had obvious degeneration,adhesion,rough surface in the livers and severe jaundice in the skin,while the livers of mice in the FSI group had no adhesion and the surface of liver was smooth,and jaundice subsided was controlled.Pathological sections showed degeneration and necrosis of live cells,and the liver tissue had inflammatory cell infiltration,collagen fibers proliferated to form pseudolobules,and the fibrosis was obviously more than that in the normal group.The liver cell structure of the FSI returned to normal,and the number of inflammatory cells in the same area was reduced.Compared with the model group,the proliferation of collagen fibers in FSI group was reduced,and the damage degree of liver lobular structure was relieved.Conclusion Flos Sophora Immaturus extract can alleviate the degree of liver fibrosis,and its mechanism is related to its anti-oxidation and improvement of abnormal liver function.