摘要
Sustainable biosynthesis of chemicals and efforts to create new molecules of interest require efficient enzymes and pathways as well as comprehensive tools and technologies to implement this rewiring.Enzymes are the key components for construction of efficient biosynthetic pathways,enzyme characterization and engineering can help to identify key enzymes and regulatory factors for construction of biosynthetic pathways,as well as improve enzyme performance;Pathway engineering can help construct biosynthetic pathways and balance metabolic network to improve biosynthetic efficiency;Tools and technologies facilitate the engineering of enzymes,pathways,and whole cells.This special issue focusing on“Pathway and Protein Engineering for Biosynthesis”comprises eight review articles and nine original research articles,which highlight and showcase current progress on Pathway and Protein Engineering and their application for biosynthesis.