油莎草须根油树脂成分及抗抑郁作用分析

Analysis of constituents and antidepressant effect of oleoresin from the fibrous roots of Cyperus esculentus L.

揭示油莎草须根油树脂抗抑郁作用及成分。采用超高效液相色谱与串联四级杆飞行时间质谱联用技术分析油莎草须根油树脂化学成分;建立慢性不可预见温和应激(chronic unpredictable mild stress,CUMS)抑郁小鼠模型,从动物行为学、神经递质含量变化、海马组织病理学考察油莎草须根油树脂抗抑郁作用。结果表明:油莎草须根油树脂含有酮类(68.891%)、酯类(8.787%)、醇类(4.258%)、烯类(0.374%)及其他物质(12.879%),主要成分为α-香附酮(59.536%)、吉马酮(5.875%)。CUMS抑郁模型小鼠给药后,油莎草须根油树脂显著缩短了小鼠强迫游泳静止时间,悬尾静止时间;显著提高了中央总路程、平均速度、中央持续时间,油莎草须根油树脂高剂量组明显提升了小鼠血清中5-羟色胺(5-HT)含量、去甲肾上腺素(NE)含量,油莎草须根油树脂高剂量组小鼠海马细胞排列整齐,神经元细胞膜基本完整。油莎草须根油树脂可能通过参与调节CUMS抑郁模型小鼠血清中5-HT和NE的表达,修复小鼠海马组织损伤来改善抑郁样行为。

To reveal the antidepressant effect and components of the fiber roots of Cyperus esculentus L.(FRCEL)oleoresinn.Ultra-performance liquid chromatography coupled with quadruple time-of-flight mass spectrometry(UPLC/Q-TOF-MS)was used to analyze the chemical composition of FRCEL oleoresin.The mouse model of chronic unpredictable mild stress(CUMS)depression was established,the antidepressant effect of FRCEL oleoresin was investigated from the aspects of animal behaviors,changes of neurotransmitter content and hippocampal histopathology.it can be concluded that FRCEL oleoresin contained ketones(68.891%),esters(8.787%),alcohols(4.258%),alkenes(0.374%)and other substances(12.879%),and the main components areα-cyperone(59.536%)and germacrone(5.875%).After CUMS model mice were administered,FRCEL oleoresin significantly shortened the rest time of forced swimming and tail suspension.The total distance,average speed and duration of the center were significantly increased.The content of 5-hydroxytryptamine(5-HT)and norepinephrine(NE)in the serum of mice were significantly increased in the high-dose FRCEL oleoresin group.The hippocampal cells of mice in the high-dose FRCEL oleoresin group were arranged neatly,and the cell membrane of neurons was basically intact.FRCEL oleoresin may improve depression-like behavior by regulating the expression of 5-HT and NE in the serum of CUMS depression model mice,repairing the damage of hippocampus in mice.