苹果中克百威残留快检测定及方法评估

Evaluation of Rapid Detection Method for Carbofuran Residues in Apples

【目的】提高苹果中克百威残留快速检测方法的灵敏度与准确度,确保苹果产品中克百威农药残留抽查的高效和准确性。【方法】以新疆阿克苏当地6个点位市售苹果为检测样品,采用行业标准NY/T 761-2008第3部分的液相色谱法为参比方法,分别选择3家快检试剂盒,根据《食品快速检测方法评价技术规范》对其快检适应性进行评估。【结果】利用参比方法和快检试剂盒均未从苹果样品中检出克百威残留。3家快检试剂盒可在0.01 mg/kg以上呈阳性显色反应,低于GB/T 2763-2019《食品安全国家标准食品中农药最大残留限量》中的限量要求;试剂盒灵敏度均为100%、特异性均为50%、假阴性率均为0、假阳性率均为50%、相对准确度均为90%。【结论】所测试剂盒适用于苹果中克百威残留量的快速筛查。

【Objective】To evaluate the adaptability of different rapid detection kits in detecting carbofuran residues in apples,improve the sensitivity and accuracy of the methods and ensure the efficiency and accuracy of selective check in apple products.【Methods】In this study,apples from six local markets in Aksu were taken as test samples,and the liquid chromatography of industry standard NY/T 761-2008 Part 3 was used as reference method.Three rapid detection kits were selected respectively,and their adaptability for rapid detection was evaluated according to Technical Specifications for Evaluation of Rapid Detection Methods of Food.【Results】None of carbofuran residues in all samples was detected by the reference method and rapid detection kits.Results in different spiked levels showed that positive color could be observed above 0.01 mg/kg,which was lower than the maximum residue limits for pesticides in food listed in GB/T 2763-2019.All rapid detection kits displayed that the sensitivity was 100%,and specificity for carbofuran residues in apples was 50%.Compared with instructions,false negative rate was 0,false positive rates were 50%and relative accuracy was 90%.【Conclusion】The rapid detection kits can be used in fast testing and screening of carbofuran residues in apples.