不同LED红、蓝光质组合对番茄灰霉病防御机制的影响

Effects of different light quality combinations of red and blue LED on tomato mold defense

为了研究不同LED红、蓝光照比例对番茄灰霉病菌丝生长及侵染力的影响,确定抑制灰霉病发展的最佳红蓝光质配比,设置5个不同的红、蓝光谱比例组合照射灰霉病病菌及接种灰霉病的番茄叶片,对灰霉病菌丝生长、病菌侵染、抗氧化保护酶活性、脯氨酸含量、丙二醛含量等相关指标进行测定。结果表明,不同红、蓝光质配比对番茄灰霉病的侵染均有一定的抑制作用,以C(红、蓝5∶1)处理下病斑抑制率最高。离体番茄叶片中SOD、POD、CAT活性随着红光比例的增加逐渐增强,以C(红、蓝5∶1)活性最强。PAL活性、脯氨酸含量也以C(红、蓝5∶1)处理最高,相比对照差异显著。丙二醛含量在C(红、蓝5∶1)处理的降低,效果最为明显。综上可知,LED红、蓝(5∶1)是对设施番茄灰霉病防治最为有利的光配比。

In this experiment,different combinations of red and blue spectra were used to irradiate Botrytis cinerea and tomato leaves inoculated with B.cinerea.The effects of different light conditions on the mycelial growth and infectivity of B.cinerea were studied,and the best red and blue light quality ratio to inhibit the development of B.cinerea were determined.SOD,POD,CAT,proline content etc,and preliminarily explore the physiological defense mechanism of different light quality ratio of red and blue led to inhibit the development of gray mold.The experiment set up 5 different treatments to measure the above relevant indexes.The results showed that different ratios of red and blue light had certain inhibitory effects on tomato gray mold infection,and the treatment with C(red and blue 5∶1)had the highest inhibition rate of lesions.The activities of SOD,POD and CAT in isolated tomato leaves increased with the increase of the ratio of red light,and the activity of C(red and blue 5∶1)was the strongest.PAL activity and proline content also showed the greatest difference between C(red and blue 5∶1)treatment compared with the control.The content of malondialdehyde in C(red and blue 5∶1)treatment had a better reduction effect,and the effect was the most obvious.In conclusion,LED(red and blue5∶1)is the most favorable light quality ratio for the prevention and control of B.cinerea in facilities.