氟啶胺在小白菜中的残留行为及膳食摄入风险评估

Residual behavior and risk assessment of dietary intake of fluazinam in pakchoi

为了建立一种由超高效液相色谱-串联质谱(UPLC-MS/MS)检测小白菜中氟啶胺的分析方法,并对小白菜中的残留量进行膳食风险评估。样品经乙腈提取,NaCl盐析、无水MgSO4、N-丙基乙二胺(PSA)和石墨化碳黑(GCB)净化,超高效液相色谱-串联质谱检测。结果表明,小白菜中氟啶胺的标准曲线为y=2 000 000 x+15 687,R2=0.992 8,平均回收率为105%~108%,相对标准偏差为1%~7%,在0.005~0.500 mg·kg-1范围内具有良好的线性关系,方法正确度和精密度均符合农作物中农药残留试验准则要求。氟啶胺的普通人群每日摄入量国家估算值为0.12 mg,占允许摄入量的19.5%,不会对一般人群构成不可接受的风险。

A method for the detection of fluazinam in pakchoi by ultra-high performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS)was established,and the dietary risk of fluazinam in pakchoi was evaluated.The pakchoi samples were extracted with acetonitrile,salted out with NaCl,purified with anhydrous MgSO4,N-propyl ethylenediamine(PSA)and graphitized carbon black(GCB),and detected by ultra performance liquid chromatography-tandem mass spectrometry.In the concentration range of 0.005-0.500 mg·kg-1,fluazinam has a good linear correlation,the standard curve was y=2000000 x+15687,with the correlation coefficient(R2)was 0.9928.And the average recovery in the range of 105%-108%,with relative standard deviation ranging from 1%to 7%.The accuracy and precision of the method were in accordance with the requirements of the guidelines for pesticide residues in crops.The national estimated daily intake of fluazinam for general population was 0.12 mg,accounting for 19.5%of the allowable intake,which does not pose an unacceptable risk to the general population.