高产酸性蛋白酶白曲霉培养条件的优化研究

Optimization of Cultivation Conditions of Aspergillus albicans with High Yield of Acid Protease

以实验室保藏的白曲霉菌株为目标菌株,从生长条件、产酶活力的关键工艺因素进行研究分析,以提高白曲霉菌株酸性蛋白酶活力为目的,探究高产酸性蛋白酶白曲霉的实验室最佳培养条件。研究结果表明,以麸皮为主要原料,培养基水分添加量为100%,培养基pH值为5,培养温度38℃,培养时间72 h,为该实验菌株的实验室最佳培养条件。

In order to improve the acid protease activity of Aspergillus albicans,the key technological factors influencing the growth conditions and enzyme activity of Aspergillus albicans were studied,and the best laboratory cultivation conditions of Aspergillus albi-cans with high yield of acid protease was explored.The best laboratory cultivation conditions were determined as follows:the main raw material was bran,the water content of the medium was 100%,the pH value of the medium was 5,the cultivation temperature was 38℃,and the cultivation time was 72 h.