角倍蚜气味结合蛋白和化学感受蛋白基因的鉴定及表达谱

Identification and expression profiling of odorant-binding protein and chemosensory protein genes in Schlechtendalia chinensis (Hemiptera: Aphididae)

【目的】通过对角倍蚜Schlechtendalia chinensis气味结合蛋白(odorant-binding proteins, OBPs)和化学感受蛋白(chemosensory proteins, CSPs)基因进行鉴定和表达谱分析,为研究OBPs和CSPs在角倍蚜嗅觉感知中的功能提供理论依据。【方法】基于角倍蚜基因组和不同发育阶段转录组数据,通过生物信息学方法鉴定和筛选角倍蚜OBPs和CSPs的基因序列;利用邻接法对角倍蚜与其他半翅目昆虫OBPs和CSPs的氨基酸序列进行系统发育分析;根据角倍蚜OBP和CSP基因在不同发育阶段(干雌、干母、春迁蚜、秋迁蚜、越冬若蚜、雌性蚜和雄性蚜)转录组数据中的TPM(tags per million)值进行表达丰度分析,结合qRT-PCR方法检测角倍蚜OBP和CSP基因在不同发育阶段的相对表达量。【结果】从角倍蚜基因组和转录组数据中鉴定出12个OBP基因(SchiOBP2-10,SchiOBP13-15)和9个CSP基因(SchiCSP1-2,SchiCSP4-10);系统发育分析发现,角倍蚜OBPs和CSPs与其他蚜虫OBPs和CSPs聚在一起形成多个同源亚群,具有可信的同源进化关系。不同发育阶段转录组表达丰度和qRT-PCR检测的时空表达谱表明,SchiOBP3/6和SchiCSP1/4/6/9/10在干母中高表达,SchiOBP4/10/14和SchiCSP2/5/7在雄性蚜中高表达,SchiOBP2在干母和雄性蚜中均高表达,SchiOBP7和SchiCSP8在春迁蚜中高表达,SchiOBP8/13/15在秋迁蚜中高表达,SchiOBP9在越冬若蚜中高表达,表明这些基因可能在角倍蚜寻找寄主植物和搜寻配偶的嗅觉识别过程中发挥着重要作用。【结论】本研究鉴定和明确了角倍蚜OBP和CSP基因及其在不同发育阶段的表达特征,为进一步研究角倍蚜嗅觉相关蛋白的功能及瘿蚜与寄主植物的化学通讯机制提供了依据。

【Aim】In order to provide theoretical basis for studying the roles of odorant-binding proteins(OBPs)and chemosensory proteins(CSPs)in olfactory recognition of Schlechtendalia chinensis by the gene identification and expression profiling of OBPs and CSPs of S.chinensis.【Methods】Based on the genome and transcriptome data of different developmental stages of S.chinensis,the gene sequences of OBPs and CSPs were identified and screened by bioinformatics methods.Phylogenetic analysis of OBPs and CSPs from S.chinensis and other Hemiptera insects based on amino acid sequences was performed using the neighbor-joining method.According to the TPM(tags per million)values of OBP and CSP genes in the transcriptome of different developmental stages(fundatrigeniae,fundatrix,spring migrant aphids,autumn migrant aphids,overwintering nymphs,sexual females and sexual males)of S.chinensis,the expression abundance was analyzed,and the relative expression levels of OBP and CSP genes were detected by qRT-PCR method.【Results】A total of 12 OBP genes(SchiOBP2-10,SchiOBP13-15)and 9 CSP genes(SchiCSP1-2,SchiCSP4-10)were identified from the genome and transcriptome data of S.chinensis.The phylogenetic analysis showed that OBPs/CSPs of S.chinensis were clustered together with OBPs/CSPs of other aphids and formed multiple homologous subgroups,indicating a credible homologous evolutionary relationship.According to the expression abundance in the transcriptome of different developmental stages and spatiotemporal expression profiles detected by qRT-PCR,SchiOBP 3/6 and SchiCSP 1/4/6/9/10 were highly expressed in fundatrix,SchiOBP 4/10/14 and SchiCSP 2/5/7 were highly expressed in sexual males,SchiOBP 2 was highly expressed in fundatrix and sexual males,SchiOBP 7 and SchiCSP 8 were highly expressed in spring migrant aphids,SchiOBP 8/13/15 were highly expressed in autumn migrant aphids,and SchiOBP 9 was highly expressed in overwintering nymphs,indicating that these genes may play important roles in the olfactory recognition of S.chinensis in searching for host plants and mates.【Conclusion】In this study,the OBP/CSP genes and their expression characteristics in different developmental stages of S.chinensis were identified and clarified,which provide a basis for further study on the function of olfactory-related proteins of S.chinensis and the chemical communication mechanism between gall aphids and their host plants.