摘要
Long-range peripheral nerve defect is a severe and worldwide disease.With the increasing development of tissue engineering,the excellent ability of nerve extracellular matrix(ECM)in peripheral nerve injury(PNI)has been widely studied and verified.Here,we present a novel microtube that contains gradient decellularized porcine sciatic nerve ECM hydrogel(pDScNM-gel)from microfluidics for sciatic nerve regeneration.The pDScNM is confirmed to enhance cell proliferation and migration,and improve the axon growth of primary dorsal root ganglions(DRGs)in a concentration-related manner.These behaviors were also achieved when cells were co-cultured in a gradient pDScNM microtube.The in vivo sciatic nerve regeneration and functional recovery were also demonstrated by assembling the gradient pDScNM microtubes with a medical silicon tube.These results indicated that the microtubes with gradient pDScNM could act as a promising alternative for repairing peripheral nerve defects and showed great potential in clinical use.
基金
National Key Research and Development Program of China(2020YFA0908200)
National Natural Science Foundation of China(81972150,82172428 and 61927805)
Natural Science Foundation of Zhejiang(LR18H150001 and LQ22E030004)
Shenzhen Fundamental Research Program(JCYJ20190813152616459)
Key scientific and technological innovation projects of Wenzhou(ZY20200023)
Wenzhou Innovation Team(Growth factor drug development)(No.201801)
CAMS Innovation Fund for Medical Sciences(2019-I2M-5-028).