南酸枣树皮药材质量标准定性研究

Quality Standard of Choerospondiatis Cortex

目的建立南酸枣树皮药材的定性质量标准。方法对药材样品进行性状鉴别,对其横切面和粉末进行显微鉴别;按2020年版《中国药典(四部)》通则方法测定药材样品水分、灰分、浸出物的含量;采用薄层色谱(TLC)法对药材样品进行定性鉴别。结果药材样品横切面落皮层多残存,内侧有数个木栓组织层带,层带内壁有增厚且木化的木栓细胞,两层带间为颓废的皮层组织,细胞壁木化;纤维束多角形,占大部分,射线3~4列细胞,近韧皮部时向一方偏斜;草酸钙方晶多散在纤维束内;多散在异形石细胞和薄壁细胞,含少数气孔。药材样品的TLC图斑点清晰,分离度高、专属性良好。11批药材样品水分含量为10.24%~10.41%,总灰分含量为3.24%~3.93%,酸不溶性灰分含量为0.26%~0.34%。50%乙醇热浸法浸出物含量为29.87%~33.58%。结论初步拟订药材样品水分含量不得过13.0%,总灰分含量不得过4.4%,酸不溶性灰分含量不得过0.4%,50%乙醇浸出物含量不少于25.0%。该研究结果可靠,可为南酸枣树皮药材质量定性标准的建立提供参考。

Objective To establish a qualitative quality standard for Choerospondiatis Cortex.Methods The characteristics of the medicinal material samples were identified,and their cross-section and powder were identified by microscopic identification.The content of water,ash,and extract in the medicinal material samples was determined according to the general principles of the Chinese Pharmacopoeia(2020 Edition,VolumeⅣ).Thin-layer chromatography(TLC)method was adopted to qualitatively identify the medicinal material samples.Results Rhytidome was mostly residual in the cross-section of the medicinal material samples,with several cork tissue layers bands on the inner side,thickened and lignified cork cells on the inner wall of the layers bands,and decayed cortical tissue between the two layer bands,and lignified cell walls.The fiber bundles were polygonal,accounting for most of them,with 3-4 rows of cells in the rays,skewed to one side when near the bast.Calcium oxalate square crystals were mostly scattered within the fiber bundles,mostly scattered in stone cells and thin-walled cells,containing few stomata.The TLC spots of the medicinal material samples were clear,with good separation and specificity.The water content of 11 batches of medicinal material samples ranged from 10.24%to 10.41%,the total ash content ranged from 3.24%to 3.93%,and the acid-insoluble ash content ranged from 0.26%to 0.34%.The extract content extracted by 50%ethanol hot leaching method ranged from 29.87%to 33.58%.Conclusion It is preliminarily proposed that the water content of the medicinal material samples should not exceed 13.0%,the total ash content should not exceed 4.4%,the acid insoluble ash content should not exceed 0.4%,and the extract content extracted by 50%ethanol should not be less than 25.0%.The study results are reliable and can provide a reference for the establishment of qualitative quality standards for Choerospondiatis Cortex.