7种防晒化妆品微生物检验方法适用性研究

Method Applicability of Microorganism Test on Seven Types of Suncare Products

目的建立化妆品微生物检验方法适用性试验方法。方法参照2015年版《化妆品安全技术规范》和2020年版《中国药典(四部)》通则1105和1106,对7种防晒化妆品进行微生物检验方法适用性考察。结果菌落总数及霉菌和酵母菌总数,1种防晒化妆品需采用1∶100(V/V)供试液消除抑菌性后进行微生物检验,其余6种均可采用常规法检验;控制菌,耐热大肠菌群、金黄色葡萄球菌和铜绿假单胞菌多可采用常规法检验,仅1种防晒化妆品需采用250 mL双倍乳糖胆盐(含中和剂)培养基检验耐热大肠菌群。结论防晒化妆品微生物检验前有必要进行方法适用性试验。

Objective To establish a test method for the applicability of microorganism test methods of suncare products.Methods According to the Safety and Technical Standards for Cosmetics(2015 Edition)and the general rules 1105 and 1106 of the Chinese Pharmacopoeia(2020 Edition,VolumeⅣ),the method applicability of the microorganism test of seven suncare products was investigated.Results The total number of bacterial colonies,molds and yeasts in one type of suncare product should be tested by 1∶100(V/V)test solution to eliminate bacteriostasis before the microorganism test,while those in the other six types could be tested by the routine method.Control bacteria,thermotolerant coliforms,Staphylococcus aureus,and Pseudomonas aeruginosa in most sunscreen products could be tested by the routine method,while the thermotolerant coliforms in one sunscreen product should be tested by 250 mL of double lactose bile salt(containing neutralizer)culture medium.Conclusion It is necessary to conduct a method applicability test before the microorganism test of sunscreen products.