circDLGAP4及miR-34c在帕金森病患者血清中的表达及临床意义

Expression and Clinical Significance of circDLGAP4 and miR-34c in the Serum of Patients with Parkinson′s Disease

目的:探讨环状RNA-DLGAP4(circular RNA-DLGAP4,circDLGAP4)及微小RNA-34c(microRNA-34c,miR-34c)在帕金森病(Parkinson′s disease,PD)患者血清中的表达及其对疾病的诊断价值。方法:选取2019年1月至2021年1月我院收治的72例PD患者作为PD组,并根据Hoehn-Yahr分级对PD患者进行分组。另选取同期在我院体检的健康志愿者75例作为对照组。通过实时荧光定量PCR检测两组受试者血清circDLGAP4及miR-34c的相对表达量,并通过统一帕金森病评定量表(UPDRS)统计PD患者UPDRSⅠ、UPDRSⅡ及UPDRSⅢ评分。通过Pearson相关性、受试者工作特征(ROC)曲线分析circDLGAP4及miR-34c在PD中的诊断价值。结果:PD组患者血清circDLGAP4和miR-34c表达水平低于对照组(P<0.05)。中度组PD患者血清circDLGAP4和miR-34c表达水平较轻度组更低(P<0.05);重度组PD患者血清circDLGAP4和miR-34c表达水平较中度组更低(P<0.05)。PD患者血清circDLGAP4和miR-34c表达水平与UPDRSⅡ评分、UPDRSⅢ评分、Hoehn-Yahr分级呈负相关(P<0.05)。血清circDLGAP4、miR-34c联合检测对PD的诊断效能更高。结论:circDLGAP4及miR-34c可作为诊断PD的分子标志物。

Objective:To investigate the expression and diagnostic value of circDLGAP4 and miR-34c in the serum of Parkinson′s disease(PD)patients.Methods:72 PD patients admitted to our hospital from January 2019 to January 2021 were selected as the PD group,and were grouped according to Hoehn-Yahr grade.In addition,75 healthy volunteers who underwent physical examination in our hospital during the same period were selected as the control group.The relative expression levels of circDLGAP4 and miR-34c in the serum of the two groups of subjects were detected by real-time quantitative PCR,and the UPDRSⅠ,UPDRSⅡand UPDRSⅢscores of PD patients were calculated by the Unified Parkinson′s Disease Rating Scale(UPDRS).The diagnostic value of circDLGAP4 and miR-34c in PD was analyzed by the method of Person correlation and receiver operating characteristic(ROC)curve.Results:The serum circDLGAP4 and miR-34c expression levels in the PD group were lower than those in the control group(P<0.05).The serum circDLGAP4 and miR-34c expression levels in moderate group Parkinson′s disease patients were lower than those in the mild group(P<0.05).The serum circDLGAP4 and miR-34c expression levels in severe group Parkinson′s disease patients were lower than those in the moderate group(P<0.05).The serum circDLGAP4 and miR-34c expression levels in PD patients were negatively correlated with UPDRSⅡscore,UPDRSⅢscore and Hoehn-Yahr grade(P<0.05).The combined detection of serum circDLGAP4 and miR-34c is more valuable in the diagnosis of PD.Conclusion:circDLGAP4 and miR-34c can be used as the molecular markers to diagnose PD.