泛素结合酶2S在胃癌中的表达及临床意义

Expression and clinical significance of UBE2S in gastric cancer

目的 探讨泛素结合酶2S(UBE2S)在胃癌(GC)组织中的表达情况,并探讨其与GC患者远期预后的关系。方法 将2016年1月—2016年12月蚌埠医学院第一附属医院胃肠外科收治的GC患者(101例)列为研究对象,收集所有患者肿瘤组织及临床信息,通过免疫组织化学法检测癌及癌旁组织中UBE2S蛋白水平,分析UBE2S与组织病理学检测值之间的关系,探讨UBE2S表达对GC预后的影响,绘制生存曲线。利用生物信息学手段分析UBE2S可能参与肿瘤发生的机制。结果 免疫组化分析显示,UBE2S在GC组织中的表达显著高于癌旁组织,与组织病理学参数的关系提示UBE2S的阳性表达与CEA、CA19-9、T分期、N分期均明显相关(均P<0.01),多因素分析提示UBE2S高表达为GC的独立危险因素(HR=2.832,95%CI:1.361~4.170;P<0.01)。Kaplan-Meier生存曲线显示,GC患者的术后生存率与UBE2S高低表达组别之间差异有统计学意义(log-rank χ^(2)=27.512,P<0.01),ROC曲线结果显示AUC=0.789,敏感度和特异度分别为77.42%和74.36%。结论 UBE2S高表达于GC中,其与GC预后密切相关,UBE2S高表达可作为影响GC患者术后生存率的独立危险因素。

Objective To investigate the expression of ubiquitin-binding enzyme 2S(UBE2S)in gastric cancer(GC)tissues,and to explore its relationship with the long-term prognosis of GC patients.Methods A total of 101 GC patients admitted to Department of Gastrointestinal Surgery,the First Affiliated Hospital of Bengbu Medical College from January 2016 to December 2016 were selected as the study subjects.Tumor tissue and clinical information of all patients were collected.UBE2S protein levels in cancer and adjacent tissues were detected by immunohistochemistry,and the relationship between UBE2S and histopathological detection values was analyzed.The effect of UBE2S expression on GC prognosis was investigated and survival curve was drawn.Bioinformatics methods were used to analyze the possible mechanism of UBE2S involved in tumor.Results Immunohistochemical analysis showed that the expression of UBE2S in GC tissues was significantly higher than that in adjacent tissues.The positive expression of UBE2S was significantly correlated with CEA,CA19-9,T stage and N stage(P<0.01).Multivariate analysis suggested that UBE2S was an independent risk factor for gastric cancer(HR=2.832,95%CI:1.361-4.170,P<0.01).Kaplan-Meier survival curve showed that the postoperative survival rate of GC patients was discrepant from different UBE2S expression(log-rankχ^(2)=27.512,P<0.01).ROC curve results showed AUC=0.789,sensitivity and specificity were 77.42%and 74.36%,respectively.Conclusion UBE2S is highly expressed in GC,which is closely related to the prognosis of GC.The high expression of UBE2S may be an independent risk factor for postoperative survival in patients with GC.