大豆LIM转录因子家族鉴定及盐胁迫响应分析

Identification of soybean LIM transcription factor family and analysis of salt stress response

为探究大豆LIM转录因子家族基因在应答盐胁迫中的功能,利用生物信息学方法,根据大豆全基因组数据,共鉴定出21个LIM基因,命名为GmLIM01~GmLIM21,分析大豆LIM基因家族的染色体位置、蛋白质的理化性质、进化关系、保守基序以及对非生物逆境胁迫的响应。结果表明,这些GmLIM基因在大豆20条染色体中的14条染色体上分布不均,片段重复是大豆LIM家族扩大的主要原因。根据系统发育进化分析,大豆21个LIM基因可分为5个亚家族,与其他9个物种的LIM基因一起构建进化树,也可分为5个亚家族。大豆的21个GmLIM基因共包含10个基序,大部分LIM蛋白含有motif 1、motif 2和motif 4这3个保守基序。此外,顺式调控元件的分析表明,在GmLIM基因的启动子序列中,与光响应、生长发育、植物激素和非生物逆境胁迫应答相关的调控元件非常丰富。定量PCR结果表明,在盐胁迫处理后大豆叶和根中GmLIM07、GmLIM17基因的表达量分别在3和6 h达到峰值,后随着处理时间的加长表达量下降。综上GmLIM基因在大豆的盐胁迫应答过程中具有重要的调控作用。

In order to explore the function of soybean LIM transcription factor family genes in salt stress response, a total of 21 LIM genes, named GmLIM01-GmLIM21, were identified based on the whole genome data of soybean using bioinformatics method. The chromosomal location, physical and chemical properties, evolutionary relationship, conservative motif of soybean LIM gene family and its response to abiotic stress were analyzed. The results showed that: These GmLIM genes were not uniformly distributed on 14 of the 20 chromosomes in soybean, and fragment duplication was the main reason for the expansion of soybean LIM family. According to phylogenetic evolution analysis, the 21 LIM genes in soybean could be divided into five subfamilies. These 21 genes could be used to construct the evolutionary tree together with LIM genes identified in nine other plant species and divided into five subfamilies. The 21 GmLIM genes in soybean contained a total of 10 motifs, and most LIM proteins contained 3 different conservative motifs, namely, motif 1, motif 2 and motif 4. In addition, the analysis of cis-regulatory elements showed that the promoter sequence of the GmLIM gene was rich in regulatory elements related to light response, growth and development, plant hormones, and abiotic stress response. The quantitative PCR results showed that after salt stress treatment the expression levels of GmLIM07 and GmLIM17 genes in both leaves and roots reached peak at 3 and 6 h, respectively, and then decreased with the prolongation of treatment time. To sum up, GmLIM genes play an important regulatory role in the process of salt stress response in soybean.