lncRNA NONRATT021477.2对氧化应激条件下BRL细胞增殖和凋亡的影响

Effects of IncRNA NONRATT021477.2 on proliferation and apoptosis of BRL cells under oxidative stress condition

以H_(2)O_(2)诱导BRL细胞建立氧化应激型模,通过ROS和MDA试剂盒检测氧化应激的发生状态。过表达和干扰表达lncRNA 77.2后,通过流式细胞术检测细胞凋亡,CCK-8法检测细胞增殖情况。结果显示,过表达lncRNA77.2后,BRL细胞内ROS和MDA含量均极显著下降(P<0.01),细胞增殖能力增强(P<0.01),细胞凋亡率下降(P<0.01);而干扰表达lncRNA77.2后,细胞内ROS显著升高(P<0.05),MDA含量极显著升高(P<0.01),细胞增殖能力下降(P<0.01),细胞凋亡率上升(P<0.01)。结果表明,lncRNA 77.2可作为抗氧化调控因子通过抑制凋亡和氧化损伤对大鼠肝细胞起到一定的保护作用。

The aim of this study was to investigate the effects of long non-coding RNA NONRATT021477.2(lncRNA 77.2)on the proliferation and apoptosis of BRL cells induced by hydrogen peroxide(H_(2)O_(2)).The BRL cells were induced by H_(2)O_(2)to establish oxidative stress model,and the occurrence state of oxidative stress was detected by ROS and MDA kit.After overexpression and interference expression of lncRNA 77.2,cell apoptosis was detected by flow cytometry,cell proliferation in BRL cells were determined by CCK8.The results showed that the over-expression of lncRNA77.2 significantly reduced the levels of ROS and MDA in BRL cells(P<0.01),enhanced the proliferation of cells(P<0.01),and decreased the apoptosis rate of cells(P<0.01).While interfering with the expression of lncRNA 77.2,ROS in BRL increased significantly(P<0.05),MDA content increased significantly(P<0.01),cell proliferation decreased(P<0.01),and apoptosis rate increased significantly(P<0.01).In conclusion,lncRNA 77.2 could be used as an antioxidant regulator to protect BRL cells by inhibiting apoptosis and oxidative damage.