摘要
In this work,we prepared a material with magnetic nanoparticles(Fe_(3)O_(4))as core,layered double hydroxides(LDHs)as affinity shell,and cerium dioxide(CeO_(2))as functional molecules(denoted as Fe_(3)O_(4)@LDH-CeO_(2)).On the basis of combined immobilized metal ion affinity chromatography(IMAC)and metal oxide affinity chromatography(MOAC),Fe_(3)O_(4)@LDH-CeO_(2) was used to enrich phosphopeptides with high efficiency.The material exhibited high selectivity(α-casein:β-casein:BSA=1:1:5000,mass ratio),high recovery(95.87%),and good reusability of 10 times adsorption-desorption experiments.The feasibility of Fe_(3)O_(4)@LDH-CeO_(2) was further investigated by extracting phosphopeptides from biological samples(nonfat milk,serum,saliva,and A549 cell lysate).
基金
National Natural Science Foundation of China(Nos.22004073 and 21961024)
Natural Science Foundation of Inner Mongolia,China(No.2020BS02010)
Talent Research Support Funds from Government-Sponsored Institution of Inner Mongolia,China(No.RCQD19002)
Inner Mongolia Minzu University Doctoral Research Startup Fund Project,China(No.BS516)
Incentive Funding from Nano Innovation Institute(NII)of Inner Mongolia Minzu University,China.
