星点设计-效应面法优化伤科活血颗粒提取工艺

Optimization of the extraction process of Shangke Huoxue Granule by central compositedesign-response surface methodology

目的优选伤科活血颗粒的提取工艺。方法以提取溶剂倍数、提取时间、提取次数为考察因素,以提取液中阿魏酸含量、芍药苷含量、干膏率为综合评价指标,结合单因素试验和星点设计-效应面法优选最佳处方工艺。结果建立的二项式拟合方程为:Y=96.16+2.42A+0.63B-3.76AB-1.57A^(2)-1.87B^(2)(P<0.01),方程模型显著。最佳提取工艺为溶剂倍数为16倍,提取2次,每次提取64 min,3批验证试验的偏差率分别为2.00%、3.23%、0.66%。结论星点设计-效应面法所建立的模型预测性高,筛选出的提取工艺稳定可行。

Objective To optimize the extraction process of Shangke Huoxue Granule.Methods Taking the factors of extraction solvent multiple,extraction time and extraction times as investigation factors,and extraction amount of ferulic acid,paeoniflorin and the ratio of extraction as comprehensive evaluation indices,one-factor experimental design and central composite design-response surface methodology were adopted to optimize the extraction process of Shangke Huoxue Granule.Results The binomial fitting equation was Y=96.16+2.42A+0.63B-3.76AB-1.57A2-1.87B2(P<0.01).The optimal extraction process parameters were confirmed to be adding 16 times of water,64 minutes each time,twice.The deviation rates between the measured values of three verification experiments and the predicted value were 2.00%,3.23%and 0.66%.Conclusion The established model of central composite design-response surface methodology has high predictability and the optimized extraction process is stable and feasible.