4种方法确诊口岸一例疟疾疑似病例应用研究

Application of Four Methods in the Diagnosis of a Suspected Malaria Case at the Port

应用疟原虫厚薄血膜涂片镜检、RDT疟原虫抗原检测、实时荧光定量PCR、微滴式数字PCR 4种检测方法对南京口岸输入的一例疟疾疑似病例进行诊断,比较分析不同检测方法的适用场景,为口岸传染病防控提供技术支持。4种检测方法结果均判断为恶性疟原虫阳性,其中微滴式数字PCR检测结果显示恶性疟原虫核酸原始浓度为157 copies/μL。结果表明,4种检测方法中以RDT抗原检测最快速、简便、综合成本最低。在病例流行病史调查明确,且有临床表现的前提下,可优先做RDT疟原虫抗原检测,其他3种方法可作为辅助检测手段。

A suspected case of malaria imported at the Nanjing port was diagnosed by four detection methods,including thick and thin blood smear microscopy,RDT malaria antigen detection,real-time fluorescent quantitative PCR and micro-drop digital PCR,and the application scenarios of different detection methods were compared and analyzed to provide technical support for the prevention and control of infectious diseases at the port.Plasmodium falciparum was positive in the four detection methods,and the original concentration of nucleic acid was 157 copies/μL in the micro-drop digital PCR detection.The results showed that the RDT antigen detection was the most rapid and simple with the lowest comprehensive cost among the four detection methods.With a clear epidemiological history and clinical manifestations of the case,RDT malaria antigen detection can be conducted first,and the other three methods can be used as auxiliary detection methods.