基于靶向代谢组学探讨两种二羧酸酰基肉碱在乙肝相关性疾病中的应用价值

Application values of two dicarboxyl⁃acylcarnitines in hepatitis B virus⁃related diseases based on targeted metabolomics

目的探讨辛二酰肉碱(suberylcarnitine,C8-DC)和十六碳二酰肉碱(hexadecanedioylcarnitine,C16-DC)诊断乙肝相关性肝细胞癌(hepatocellular carcinoma,HCC)和肝硬化(liver cirrhosis,LC)的价值。方法收集2018年5月至2019年5月于广西医科大学附属肿瘤医院就诊的41例HCC患者(HCC组)、18例LC患者(LC组)的血清,另选择30名同期于我院就诊的健康志愿者作为正常对照组(NC组)。采用超高效液相色谱-四极杆飞行时间串联质谱联用技术对C8-DC和C16-DC进行靶向分析。采用受试者工作特征(receiver operating characteristic,ROC)曲线评价诊断效能。结果LC组和HCC组的C8-DC水平高于NC组(均P<0.001);LC组C16-DC水平高于NC组(P<0.001)和HCC组(P<0.05)。C8-DC诊断LC、HCC及区分LC和HCC的ROC曲线下面积(area under curve,AUC)分别为0.889、0.776和0.654;C16-DC诊断LC、HCC及区分LC和HCC的AUC分别为0.878、0.646和0.743。C8-DC和C16-DC联合诊断LC、HCC及区分LC和HCC的AUC分别为0.898、0.797和0.714。结论C8-DC和C16-DC与乙肝相关性HCC和LC密切相关,对诊断乙肝相关性HCC和LC有一定的价值。

Objective To investigate the values of suberylcarnitine(C8‑DC)and hexadecanedioylcarnitine(C16‑DC)in the diagnosis of hepatitis B virus(HBV)‑related hepatocellular carcinoma(HCC)and liver cirrhosis(LC).Methods The serum of 41 HCC patients(HCC group)and 18 LC patients(LC group)from Guangxi Medical University Cancer Hospital from May 2018 to May 2019 were collected,and 30 healthy volunteers during the same period were selected as the normal control group(NC group).The targeted analysis of C8‑DC and C16‑DC was performed by using the combined technology of ultra‑high performance liquid chromatography and quadrupole timeof‑flight mass spectrometry.The receiver operating characteristic(ROC)curve was used to evaluate the diagnostic efficacy.Results The levels of C8‑DC in the LC group and HCC group were higher than those in the NC group(all P<0.001),and the levels of C16‑DC in the LC group were higher than those in the NC group(P<0.001)and HCC group(P<0.05).The areas under the ROC curve(AUC)of C8‑DC for diagnosing and discriminating LC and HCC were 0.889,0.776 and 0.654,respectively;the AUC of C16‑DC for diagnosing and discriminating LC and HCC were 0.878,0.646 and 0.743,respectively;the AUC of combining C8‑DC and C16‑DC for diagnosing and discriminating LC and HCC were 0.898,0.797 and 0.714,respectively.Conclusions C8‑DC and C16‑DC are closely related to HBVrelated HCC and LC,and have certain value in diagnosing HBV‑related HCC and LC.