草酸青霉复配羟基磷灰石对萝卜生长和土壤代谢活性的影响

Effects of Penicillium oxalate combined with hydroxyapatite on the radish growth and soil metabolic activity

由于传统磷肥的不可持续性和二次污染,亟待开发新型绿色磷肥.本研究将草酸青霉与羟基磷灰石联合使用,构建微生物-无机磷矿的复配体系,通过盆栽试验探讨该体系对萝卜幼苗生长特性和土壤代谢活性的影响.结果表明,萝卜幼苗经复配处理后的根、茎长、叶片叶绿素含量分别显著增加46.8%、31.8%和50.4%,根系活力提高至空白处理的1.49倍,萝卜生长状况明显改善.接种草酸青霉将复配体系中柠檬酸可提取磷和酶矿化磷分别降低5.5%和31.9%,可能是萝卜和微生物对其同化加强.复配处理有效地提高土壤有机质和有效磷含量,与萝卜幼苗生长特性显著正相关.此外,复配处理显著提高的土壤代谢物中的糖类含量,有益于植物根际与土壤微生物之间物质交换和互惠.本研究为草酸青霉联合羟基磷灰石作为新型生物磷肥的前景提供理论依据.

Owing to the unsustainability and secondary pollution of traditional P fertilizers,and novel green P fertilizers need to be developed urgently.In this study,the phosphate solubilizing fungi Penicillium oxalicum was used in combination with hydroxyapatite to explore the effects on the growth status of radish seeding and soil metabolism via pot experiments.Our results indicated that the highest length of root and stem,and chlorophyll levels of leaves were in the combined treatment,which were significantly increased by 46.8%,31.8%and 50.4%,respectively,and the root activity was increased by 1.49-fold of CK,thus the growth status of radish seedlings improved significantly.Penicillium oxalicum reduced citric extractable P and enzyme mineralized P in combined treatment by 5.5%and 31.9%,implied that the enhanced P assimilation of radish and microorganisms.The combined treatment also effectively elevated soil organic matter and available P content,which was significantly positively correlated with the growth characteristics of radish seedlings.Moreover,the material exchange and reciprocity between plant rhizosphere and soil microorganisms could profited from the increase in soil sugars.This study provided the theoretical basis for the prospective of Penicillium oxalate combined with hydroxyapatite as a novel bio-phosphate fertilizer.