PABPC1对人单核白血病细胞系THP-1翻译活性的影响

Influence of PABPC1 on translation activity in human monocytic leukemia cell line THP-1

目的研究细胞质多聚腺苷酸结合蛋白-1(PABPC1)对人单核细胞白血病细胞系THP-1翻译活性的影响。方法在THP-1细胞中转染shPABPC1,Western blot验证敲低效率,多聚核糖体分析技术检测细胞翻译活性变化。结果转染后THP-1细胞中PABPC1表达被抑制(P<0.001);与对照组相比,敲低PABPC1组的翻译活性减弱。结论敲低PABPC1可抑制THP-1细胞翻译活性,PABPC1可能成为治疗急性髓系白血病(AML)的潜在靶点。

Objective To investigate the effect of cytoplasmic poly(A)binding protein-1(PABPC1)on translation of human monocytic leukemia cells line THP-1.Methods Specific short hairpin RNA(shRNA)was transfected into THP-1 cells.Western blot was used to verify the interference efficiency.The polysome profiling was used to examine the translation ability of THP-1 cells.Results The expression of PABPC1 protein was decreased in shPABPC1 transfected THP-1(P<0.05).Compared with control group,the translation ability of THP-1 cells in shPABPC1 group was reduced.Conclusions Knockdown of PABPC1 may inhibit the translation in THP-1 cells and PABPC1 is a potential target for the treatment of acute myeloid leukemia(AML).