腹泻病例中致泻性大肠埃希菌的病原学特征分析

Analysis of the Etiological Characteristics of Diarrheogenic Escherichia coli in Diarrhea Cases

目的:通过毒力基因检测,了解腹泻病例中致泻性大肠埃希菌(DEC)的分布特点,为DEC的防治提供依据。方法:收集1198例腹泻患者粪便中分离的大肠埃希菌,用毛细管电泳技术进行毒力基因检测,确定其致病型别并分析其分布特点。结果:1198例腹泻病例共检出DEC 92株,阳性率为7.68%。其中肠聚集性大肠埃希菌(EAEC)60株,占阳性菌的65.22%;肠产毒性大肠埃希菌(ETEC)20株,占阳性菌的21.74%;肠致病性大肠埃希氏菌(EPEC)10株,占阳性菌的10.87%;肠出血性大肠埃希菌(EHEC)和肠侵袭性大肠埃希菌(EIEC)各1株,分别占阳性菌的1.09%。结论:1198例腹泻患者中EAEC为主要DEC型别,以典型EAEC为主;肠致病性大肠埃希菌(EPEC)主要为非典型的EPEC。

Objective:Through virulence gene testing,the distribution characteristics of diarrheogenic Escherichia coli(DEC)in diarrhea cases were understood,which provided a basis for the prevention and treatment of DEC.Method:Escherichia coli isolated from the feces of 1198 patients with diarrhea was collected,and virulence gene detection was performed by capillary electrophoresis to determine its pathogenic type and analyze its distribution characteristics.Result:A total of 92 DEC strains were detected in 1198 cases of diarrhea,with a positive rate of 7.68%.Among them,there were 60 strains of enteroaggregative Escherichia coli(EAEC),accounting for 65.22%of the positive bacteria;there were 20 strains of enterotoxic Escherichia coli(ETEC),accounting for 21.74%of the positive bacteria;10 strains of enteropathogenic Escherichia coli(EPEC),accounting for 10.87%of the positive bacteria;there was one strain each of enterohaemorrhagic Escherichia coli(EHEC)and enterinvasive Escherichia coli(EIEC),accounting for 1.09%of the positive bacteria,respectively.Conclusion:In 1198 patients with diarrhea,EAEC was the main DEC type,mainly typical EAEC,and enteropathogenic Escherichia coli(EPEC)was mainly atypical EPEC.