响应面优化皂角刺皂苷超声波辅助提取工艺及其降血糖活性

Optimization of Ultrasonic-assisted Extraction Technology of Saponins from Gleditsia sinensis Thorn by RSM and Its Hypoglycemic Activity

在单因素试验基础上,采用响应面法优化超声波辅助提取皂角刺皂苷的最佳工艺,并考察了最佳提取工艺下提取物对α-葡萄糖苷酶和α-淀粉酶的抑制活性。实验结果表明:皂角刺总皂苷超声波辅助提取的最佳工艺为液料比16∶1(mL∶g)、乙醇体积分数60%、超声波时间80 min和提取温度50℃,在此条件下皂角刺总皂苷得率为13.28%±0.25%。最佳工艺下的提取物对α-葡萄糖苷酶的半数抑制质量浓度(IC 50)值为(0.146±0.019)g/L,强于阳性对照阿卡波糖IC 50值((0.48±0.18)g/L);当提取物质量浓度为2 g/L时,对α-淀粉酶抑制率可达35.13%±0.58%,表明皂角刺具有开发成为Ⅱ型糖尿病药物的潜力。

On the basis of single factor experiment,response surface methodology(RSM)was used to optimize the ultrasonic-assisted extraction optimum process of saponins from Gleditsia sinensis Thorn(GST),and the inhibitory activities of the extracts onα-glucosidase andα-amylase were investigated under the optimal extraction conditions.The results showed that the optimal ultrasonic-assisted extraction conditions were solid-liquid ratio of 16∶1(mL∶g),ethanol volume fraction of 60%,ultrasonic time of 80 min and extraction temperature of 50℃.Under these conditions,the yield of total saponins was 13.28%±0.25%,and the IC 50 value ofα-glucosidase was(0.146±0.019)g/L,which was stronger than that of positive control acarbose with the median inhibitory concentration(IC 50)value of(0.48±0.18)g/L.When the mass concentration of the extract was 2 g/L,the inhibition rate ofα-amylase could reach 35.13%±0.58%,indicating that GST had the potential development into a drug for type II diabetes.